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Dengue virus prM protein antibody

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093

Cat. No. GTX128093

Reference 15
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Dengue virus 2
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus prM protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

Product Note

This antibody was raised against Dengue virus 2 prM protein, which may cross react with prM protein of Dengue virus 1/3/4 but does not recognize with prM protein of JEV.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus PrM protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885700

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Premembrane , Dengue Virus PrM , DENV Premembrane , DENV PrM

Research Area

DATA IMAGES

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093

GTX128093 WB Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093

GTX128093 WB Image

prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
A.30 μg BHK-21 whole cell lysate/extract
B.30 μg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
C.30 μg whole cell lysate/extract of JEV virus infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093

GTX128093 WB Image

The data was published in the journal PLoS Pathog in 2013. PMID: 24348242

REFERENCE

Application Reference

K Tabata et al. Appl Microbiol Biotechnol 2023; Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
Brugier A et al. J Virol 2022; 96 (7) : e0196221 RACK1 Associates with RNA-Binding Proteins Vigilin and SERBP1 to Facilitate Dengue Virus Replication.
Application : WB
Ponndorf D et al. Plant Biotechnol J 2021; 19 (4) : 745-756 Plant-made dengue virus-like particles produced by co-expression of structural and non-structural proteins induce a humoral immune response in mice.
Lin JJ et al. PLoS Pathog 2021; 17 (3) : e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
Application : WB
Labeau A et al. J Virol 2020; 94 (7) A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.
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Submit a Reference

REVIEW

Dengue virus prM protein antibody Cat. No. GTX128093

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
Date : Anonymous submitted on 25-Dec-2019
Score :
Application Tested : WB
Sample Species : DENV2
Sample : Huh7 cells
Amount used : 10 μg
Blocking : 2.5% non-fat skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Huh7 whole cell lysate; Lane 2: Dengue virus type II NGC strain infected.
Submit a Review
Application Reference
K Tabata et al. Appl Microbiol Biotechnol 2023; Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
Brugier A et al. J Virol 2022; 96 (7):e0196221 RACK1 Associates with RNA-Binding Proteins Vigilin and SERBP1 to Facilitate Dengue Virus Replication.
Application : WB
Ponndorf D et al. Plant Biotechnol J 2021; 19 (4):745-756 Plant-made dengue virus-like particles produced by co-expression of structural and non-structural proteins induce a humoral immune response in mice.
Lin JJ et al. PLoS Pathog 2021; 17 (3):e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
Application : WB
Labeau A et al. J Virol 2020; 94 (7) A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.
Butler, M et al. Viruses 2020; 12 (6):E654 Cyclin-Dependent Kinases 8 and 19 Regulate Host Cell Metabolism During Dengue Virus Serotype 2 Infection.
Application : WB
Xie X et al. Cell Host Microbe 2019; 26 (5):606-622.e8 Dengue NS2A Protein Orchestrates Virus Assembly.
Application : WB
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Chie Aoki-Utsubo et al. Bio Protoc 2018; 8 (10):e2855 Virucidal and Neutralizing Activity Tests for Antiviral Substances and Antibodies.
H?l?ne Boigard et al. Vaccine 2018; 36 (50):7728-7736 Dengue-2 virus-like particle (VLP) based vaccine elicits the highest titers of neutralizing antibodies when produced at reduced temperature.
Application : WB
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
Application : WB
Clark KB et al. Virology 2016; 493:162-172 Characterization of dengue virus 2 growth in megakaryocyte-erythrocyte progenitor cells.
Application : WB
Tham HW et al. J Infect Dev Ctries 2015; 9 (12):1338-49 Protein-protein interactions between A. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cDNA library.
Application : ICC/IF
Mukherjee S et al. J Virol 2014; The mechanism and significance of cell type-dependent neutralization of flaviviruses.
Application : WB
VanBlargan LA et al. PLoS Pathog 2013; 9 (12):e1003761 The type-specific neutralizing antibody response elicited by a dengue vaccine candidate is focused on two amino acids of the envelope protein.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169