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Fumarate hydratase antibody [N2C2], Internal

KO/KD Orthogonal Overexpression
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110128

Cat. No. GTX110128

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Fumarate hydratase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

mouse liver , 293T , A431 , rat liver

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950282

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1

Cellular Localization

Mitochondrion , Cytoplasm

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein by western blot analysis.
A.50 μg rat liver lysate/extract
10% SDS-PAGE
Fumarate hydratase antibody [N2C2], Internal (GTX110128) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431 (GTX27909)
10% SDS PAGE
GTX110128 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110128

GTX110128 ICC/IF Image

Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Fumarate hydratase antibody [N2C2], Internal detects FH protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
10% SDS-PAGE
Fumarate hydratase antibody [N2C2], Internal (GTX110128) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110128

GTX110128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using fumarate hydratase (GTX110128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Vadhan A et al. Oncol Lett 2023; 25 (1) : 42 Fumarate hydratase inhibits non-small cell lung cancer metastasis via inactivation of AMPK and upregulation of DAB2.
Application : WB, IHC-P
Reactivity : Human
Lee SY et al. Oncol Rep 2021; 45 (4) Bcl2l10 induces metabolic alterations in ovarian cancer cells by regulating the TCA cycle enzymes SDHD and IDH1.
Application : WB
Reactivity : Human
Tseng CW et al. Cancer Res 2018; (Epub) Transketolase regulates the metabolic switch to control breast cancer cell metastasis via the alpha-ketoglutarate signaling pathway.
Application : WB
Reactivity : Human
Shanmugasundaram K et al. J Biol Chem 2014; The Oncometabolite Fumarate Promotes Pseudohypoxia Through Noncannonical Activation of NF-κB Signaling.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169