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alpha 1 Catenin (phospho Ser641) antibody

Orthogonal
Anti-alpha 1 Catenin (phospho Ser641) antibody used in Western Blot (WB). GTX79145

Cat. No. GTX79145

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Dog
Package
100 μg ($399)
See all alpha 1 Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human α-catenin around the phosphorylation site of serine 641 (D-D-Sp-D-F).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11175620

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CAP102,CTNNA1,MDPT2,catenin alpha 1,alpha 1 Catenin

Cellular Localization

Cytoplasm,Cytoskeleton,Cell junction,Adherens junction,Cell membrane

Background

This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-alpha 1 Catenin (phospho Ser641) antibody used in Western Blot (WB). GTX79145

GTX79145 WB Image

Orthogonal

WB analysis of A431 cells untreated or treated with EGF (200ng/ml, 30min) lysates using GTX79145 alpha 1 Catenin (phospho Ser641) antibody (Lane 3 and 4) and alpha 1 Catenin antibody (Lane 1 and 2).

REFERENCE

Application Reference

Li X et al. Mol Cell 2018; 72 (4) : 650-660.e8 Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication.
Application : WB
Reactivity : Mouse
Escobar DJ et al. J Cell Sci 2015; 128 (6) : 1150-65 α-Catenin phosphorylation promotes intercellular adhesion through a dual-kinase mechanism.
Application : ICC/IF
Reactivity : Dog
Submit a Reference

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Package List Price ($)
$ 399