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GFP antibody

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970
Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970
Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX13970

Cat. No. GTX13970

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Application

WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC, IHC (Free Floating)

Reactivity

Species independent
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:10000
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
ELISA Assay dependent
IHC 1:2000-1:5000
IHC (Free Floating) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Sodium Phosphate (10 mM) buffered isotonic saline (0.9%), 50% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant.

Purification

Purified by antigen-affinity chromatography
Serile filtered.

Conjugation

Unconjugated

RRID

AB_371416

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Green Fluorescent Protein

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

IHC analysis of paraformaldehyde-fixed (4%) neonatal mouse brain using GTX13970 GFP antibody.

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

Anti-GFP antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX13970

GTX13970 IHC (Free Floating) Image

The data was published in the 2017 in Cell. PMID: 28041852

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

The data was published in the journal Sci Rep in 2017. PMID: 28959031

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

The data was published in the journal Elife in 2014. PMID: 25490154

Anti-GFP antibody used in IHC (Whole mount) (IHC-Wm). GTX13970

GTX13970 IHC-Wm Image

The data was published in the journal Elife in 2017.PMID: 28832322

REFERENCE

REVIEW

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Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399