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Ku70 + Ku80 antibody [162]

Anti-Ku70 + Ku80 antibody [162] used in Western Blot (WB). GTX23108

Cat. No. GTX23108

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 162

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, IP, EMSA, RIP

Reactivity

 Human, Mouse, Rat, Monkey, Xenopus laevis
Package
500 μl ($399)
See all Ku70 + Ku80 products

APPLICATION

Application Note

Flow Cytometry / Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2μg/mg protein lysate. Immunohistology (formalin fixed paraffin embedded): use at a concentration 1 - 2μg/ml for 30 min at RT (antigen retrieval is not required). Optimal dilutions / concentrations should be determined by the end user. This antibody is not suitable for Western blot.

Positive Control

HeLa cells. Tonsil.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human B cell nuclei from plasmacytoid 2p68 cells.

Purification

Purified by ammonium sulfate precipitation.

Conjugation

Unconjugated

RRID

AB_384945

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CTC75 , FLJ39089 , CTCBF , KARP-1 , G22P1 , KARP1 , KU70 , KU80 , ML8 , KUB2 , TLAA , Ku86 , NFIV , KARP 1

Cellular Localization

Nuclear.

Background

The Ku autoantigen is a heterodimer of 70 kDa (p70) and ~80 kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Research Area

DATA IMAGES

Anti-Ku70 + Ku80 antibody [162] used in Western Blot (WB). GTX23108

GTX23108 WB Image

The data was published in the journal Nucleic Acids Res in 2014.PMID: 24589584

REFERENCE

Application Reference

Kawata M et al. J Oral Pathol Med 2017; Additive effect of radiosensitization by 2-deoxy-D-glucose delays DNA repair kinetics and suppresses cell proliferation in oral squamous cell carcinoma.
Application : WB
Reactivity : Human
Lamaa A et al. EMBO Rep 2016; 17 (4) : 508-18 A novel cytoprotective function for the DNA repair protein Ku in regulating p53 mRNA translation and function.
Application : RIP
Reactivity : Human
Maiti AK et al. Hum Mol Genet 2014; 23 (15) : 4161-76 Combined protein- and nucleic acid-level effects of rs1143679 (R77H), a lupus-predisposing variant within ITGAM.
Application : EMSA
Reactivity : Human
Morales JC et al. Nucleic Acids Res 2014; Kub5-Hera, the human Rtt103 homolog, plays dual functional roles in transcription termination and DNA repair.
Application : WB
Reactivity : Mouse
Masuda Y et al. Biochem Biophys Res Commun 2011; 415 (2) : 305-12 Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399