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Collagen I antibody [COL-1]

Orthogonal
Anti-Collagen I antibody [COL-1] used in IHC (Paraffin sections) (IHC-P). GTX26308
Anti-Collagen I antibody [COL-1] used in IHC (Frozen sections) (IHC-Fr). GTX26308
Anti-Collagen I antibody [COL-1] used in IHC (Frozen sections) (IHC-Fr). GTX26308
Anti-Collagen I antibody [COL-1] used in Western Blot (WB). GTX26308
Anti-Collagen I antibody [COL-1] used in IHC (Paraffin sections) (IHC-P). GTX26308
Anti-Collagen I antibody [COL-1] used in IHC (Paraffin sections) (IHC-P). GTX26308

Cat. No. GTX26308

Reference 61

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 COL-1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, Dot, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Pig, Deer
Package
100 μl ($399)
See all Collagen I products
PRODUCT

Summary

Collagen I antibody (Collagen 1 antibody) detects type I collagen, a fibrillar type collagen found in almost all connective tissues. The main isoform of type I collagen is a heterotrimer of two α1 and one α2 chains subject to significant posttranslational modification and crosslinking. Mutations in type I collagen are found in osteogenesis imperfecta and Ehlers-Danlos syndrome, but this collagen is also linked to other connective tissue disease as well as disorders associated with aging.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:2000
IP Assay dependent
Dot Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Product Note

The antibody is reactive with the native (helical) form of collagen type I in ELISA and dot blot. The product is not reactive when tested on thermally denatured molecules. In immunohistochemical staining of acetone-fixed and unfixed frozen sections, a strong staining of connective tissue fibers is seen. Using the dot blot and ELISA techniques, the antibody shows no cross-reactivity with collagen types, II, III, IV, V, VI, VII, IX, X and XI. The epitope recognized by the antibody is sensitive to routine formalin fixation and paraffin embedding.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Bovine

Immunogen

bovine skin collagen type I

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385204

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type I alpha 1 chain

Background

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Database

Research Area

DATA IMAGES

Anti-Collagen I antibody [COL-1] used in IHC (Paraffin sections) (IHC-P). GTX26308

GTX26308 IHC-P Image

Collagen I antibody [COL-1] detects Collagen I protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rabbit tongue.
Collagen I stained by Collagen I antibody [COL-1] (GTX26308) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Collagen I antibody [COL-1] used in IHC (Frozen sections) (IHC-Fr). GTX26308

GTX26308 IHC-Fr Image

IHC-Fr analysis of human tongue tissue using GTX26308 Collagen I antibody [COL-1] at 1:2,000.

Anti-Collagen I antibody [COL-1] used in IHC (Frozen sections) (IHC-Fr). GTX26308

GTX26308 IHC-Fr Image

IHC-Fr analysis of pig tongue tissue using GTX26308 Collagen I antibody [COL-1] at 1:4,000.

Anti-Collagen I antibody [COL-1] used in Western Blot (WB). GTX26308

GTX26308 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2020. PMID: 32235811

Anti-Collagen I antibody [COL-1] used in IHC (Paraffin sections) (IHC-P). GTX26308

GTX26308 IHC-P Image

The data was published in the journal Biomed Res Int in 2015. PMID: 25918720

Anti-Collagen I antibody [COL-1] used in IHC (Paraffin sections) (IHC-P). GTX26308

GTX26308 IHC-P Image

The data was published in the journal J Orthop Surg Res in 2019.PMID: 31272458

REFERENCE

Application Reference

W Bo et al. Adv Sci (Weinh) 2024; : e2305581 Manipulation of Glutamatergic Neuronal Activity in the Primary Motor Cortex Regulates Cardiac Function in Normal and Myocardial Infarction Mice
YM Akbar et al. Eur J Dent 2024; Hyperbaric Oxygen Therapy to Minimize Orthodontic Relapse in Rabbits
SL Lu et al. Research Square 2023; Measurement of ECM stiffness and liver cancer radiosensitivity using shear- wave-based elasticity in millimeter-sized 3D culture models
Huang L et al. Signal Transduct Target Ther 2023; 8 (1) : 417 Immunity-and-matrix-regulatory cells enhance cartilage regeneration for meniscus injuries: a phase I dose-escalation trial.
Pei YA et al. Mater Today Bio 2023; 23 : 100805 The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation.
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REVIEW

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Application Reference
W Bo et al. Adv Sci (Weinh) 2024;:e2305581 Manipulation of Glutamatergic Neuronal Activity in the Primary Motor Cortex Regulates Cardiac Function in Normal and Myocardial Infarction Mice
YM Akbar et al. Eur J Dent 2024; Hyperbaric Oxygen Therapy to Minimize Orthodontic Relapse in Rabbits
SL Lu et al. Research Square 2023; Measurement of ECM stiffness and liver cancer radiosensitivity using shear- wave-based elasticity in millimeter-sized 3D culture models
Huang L et al. Signal Transduct Target Ther 2023; 8 (1):417 Immunity-and-matrix-regulatory cells enhance cartilage regeneration for meniscus injuries: a phase I dose-escalation trial.
Pei YA et al. Mater Today Bio 2023; 23:100805 The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation.
YA Pei et al. Mater Today Bio 2023;:100805 The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation
Gu X et al. Exp Ther Med 2023; 26 (3):413 1,25(OH)(2)D(3) ameliorates doxorubicin?induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress.
XIN GU et al. Exp Ther Med 2023; 26:413 1,25(OH)?D? ameliorates doxorubicin?induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress
Application : WB
Zhou Y et al. Regen Ther 2023; 23:25-36 Polydopamine-coated biomimetic bone scaffolds loaded with exosomes promote osteogenic differentiation of BMSC and bone regeneration.
Application : ICC/IF
Reactivity : Rabbit
Pei M et al. Bioact Mater 2023; 23:353-367 Matrix from urine stem cells boosts tissue-specific stem cell mediated functional cartilage reconstruction.
Application : IHC
Reactivity : Rabbit
Takaya K et al. Cells 2022; 11 (23) Aging Fibroblasts Adversely Affect Extracellular Matrix Formation via the Senescent Humoral Factor Ependymin-Related Protein 1.
Application : WB
Reactivity : Human
Ko S et al. Am J Cancer Res 2022; 12 (9):4326-4342
Pei YA et al. Appl Sci (Basel) 2022; 12 (1) Hypoxia Modulates Regenerative Potential of Fetal Stem Cells.
Application : IHC-P
Reactivity : Human
Cariati I et al. Int J Mol Sci 2022; 23 (18) Whole Body Vibration Improves Brain and Musculoskeletal Health by Modulating the Expression of Tissue-Specific Markers: FNDC5 as a Key Regulator of Vibration Adaptations.
Xiao W et al. Sci Adv 2022; 8 (28):eabn1958 Recombinant DTβ4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93+/CD34+ cells for endothelialization.
Alexandr Kurkov et al. PLoS One 2021; 16 (1):e0245159 Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum.
Application : IHC-P
Reactivity : Human
Alexey Fayzullin et al. Biology (Basel) 2021; 10 (2) Modeling of Old Scars: Histopathological, Biochemical and Thermal Analysis of the Scar Tissue Maturation.
Application : IHC-Fr
Reactivity : Rabbit
Guozong Wang et al. Am J Transl Res 2021; 13 (3):967-978 Core decompression combined with implantation of β-tricalcium phosphate modified by a BMSC affinity cyclic peptide for the treatment of early osteonecrosis of the femoral head.
Application : IHC-P
Reactivity : Rabbit
Kai-Chieh Chou et al. Polymers (Basel) 2021; 13 (20) Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model.
Application : IHC-P
Reactivity : Rat
Lam HYP et al. PLoS Negl Trop Dis 2021; 15 (6):e0009554 Ameliorative effects of Schisandrin B on Schistosoma mansoni-induced hepatic fibrosis in vivo.
Application : WB
Reactivity : Mouse
Song G. et al. Materials & Design 2021; 199:109428 In vitro extracellular matrix deposition by vascular smooth muscle cells grown in fibroin scaffolds, and the regulation of TGF-β1.
Application : IHC-P
Ghorbani F et al. Biotechnol Prog 2020;:e3043 Decoration of electrical conductive polyurethane-polyaniline/polyvinyl alcohol matrixes with mussel-inspired polydopamine for bone tissue engineering.
Kuzmanov U et al. Nat Biomed Eng 2020; 4 (9):889-900 Mapping signalling perturbations in myocardial fibrosis via the integrative phosphoproteomic profiling of tissue from diverse sources.
Application : ICC/IF
Wang C et al. Biotechnol Lett 2020; 42 (8):1567-1579 Mechanical injury and IL-1β regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes.
Application : WB
Reactivity : Human
Lai CC et al. Int J Mol Sci 2020; 21 (6) Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells.
Application : WB
Reactivity : Human
Chen S et al. J Cell Biochem 2020; (Epub) M3 receptor modulates extracellular matrix synthesis via ERK1/2 signaling pathway in human bladder smooth muscle cells.
Application : WB
Reactivity : Human
Song SM et al. Mater Sci Eng C Mater Biol Appl 2020; 111:110783 A bi-phase core-shell structure of Mg-based bulk metallic glass for application in orthopedic fixation implants.
Application : WB
Reactivity : Human
Chen C et al. Am J Sports Med 2020;:363546519898519 Repair of a Meniscal Defect in a Rabbit Model Through Use of a Thermosensitive, Injectable, In Situ Crosslinked Hydrogel With Encapsulated Bone Mesenchymal Stromal Cells and Transforming Growth Factor 帣1.
Application : IHC-P
Reactivity : Rabbit
Liu X et al. Int J Nanomedicine 2019; 14:457-468 Biocompatibility evaluation of antibacterial Ti-Ag alloys with nanotubular coatings.
Mori S et al. Nat Commun 2019; 10 (1):3802 Self-organized formation of developing appendages from murine pluripotent stem cells.
Application : IHC-Fr
Reactivity : Mouse
Lin PH et al. Cancers (Basel) 2019; 11 (8) Isoliquiritigenin Suppresses E2-Induced Uterine Leiomyoma Growth through the Modulation of Cell Death Program and the Repression of ECM Accumulation.
Application : WB
Reactivity : Rat
Wang G et al. J Orthop Surg Res 2019; 14 (1):204 BMSC affinity peptide-functionalized β-tricalcium phosphate scaffolds promoting repair of osteonecrosis of the femoral head.
Application : IHC-P
Reactivity : Rat
Gu J et al. Evid Based Complement Alternat Med 2019; 2019:1358469 Huangqi Shengmai Yin Protects against Radiation-Induced Cardiac Fibrosis Injury by Regulating the TGF-帣1/Smads and MMPs.
Application : IHC-P
Reactivity : Rat
Rodr?guez MA et al. Exp Eye Res 2019; 182:182-193 Gene expression profile of extraocular muscles following resection strabismus surgery.
Application : IHC-Fr
Reactivity : Rabbit
Svistushkin MV et al. J Microsc 2019; 274 (1):55-68 Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study.
Application : IHC-P
Tsai CH et al. Materials (Basel) 2019; 12 (2) Improved Bioactivity of 3D Printed Porous Titanium Alloy Scaffold with Chitosan/Magnesium-Calcium Silicate Composite for Orthopaedic Applications.
McLoon LK et al. Invest Ophthalmol Vis Sci 2018; 59 (1):322-329 Composition, Architecture, and Functional Implications of the Connective Tissue Network of the Extraocular Muscles.
Li X et al. J Biomed Mater Res A 2018; 106 (12):3222-3230 Suppressing inflammation and enhancing osteogenesis using novel CS-EC@Ca microcapsules.
Application : IHC-P
Reactivity : Rabbit
Chuang HM et al. Int J Mol Sci 2018; 19 (10) Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide.
Application : WB
Reactivity : Human
Chao PY et al. Sci Rep 2018; 8 (1):14470 Shear Wave Elasticity Measurements of Three-Dimensional Cancer Cell Cultures Using Laser Speckle Contrast Imaging.
Application : IP
Fang CC et al. Sci Rep 2018; 8 (1):13802 AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage.
Application : IHC-P
Reactivity : Mouse
Im H et al. Tissue Eng Part A 2018; (Epub) Skin regeneration with a scaffold of predefined shape and bioactive peptide hydrogels.
Application : IHC-P
Reactivity : Mouse
Kim BS et al. Biomaterials 2018; (Epub) 3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering.
Application : IHC-P
Reactivity : Pig
Shentu TP et al. Sci Rep 2017; 7 (1):18052 Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation.
Application : WB
Reactivity : Human
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
Application : WB
Reactivity : Human
Santos SAAD et al. Biochem Biophys Res Commun 2017; 493 (4):1581-1586 Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity.
Application : IHC-Fr
Reactivity : Rat
Campbell TM et al. Bone 2017; 106:42-51 Bone replaces articular cartilage in the rat knee joint after prolonged immobilization.
Application : IHC-P
Reactivity : Rat
Tai BCU et al. Biotechnol J 2017; (Epub) Synthetic Poly(vinylalcohol)-based Membranes for Cartilage Surgery and Repair.
Application : IHC-P
Reactivity : Rabbit
Delella FK et al. Cell Biol Int 2017; (Epub) Fibrillar Collagen Genes are Not Co-ordinately Up-Regulated with TGF Β1 Expression in Finasteride-Treated Prostate.
Application : IHC-Fr
Reactivity : Rat
Kim JE et al. Tissue Eng Part A 2017; (Epub) Skin regeneration with self-assembled peptide hydrogels conjugated with substance P in a diabetic rat model.
Application : IHC-P
Reactivity : Rat
Li Y et al. Xenotransplantation 2017; (Epub) Construction of bioengineered hepatic tissue derived from human umbilical cord mesenchymal stem cells via aggregation culture in porcine decellularized liver scaffolds.
Application : IHC-P
Reactivity : Pig
Bao J et al. Int J Mol Med 2016; 38 (2):457-65 Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold.
Application : IHC-P
Reactivity : Pig
Ganai AA et al. Chem Biol Interact 2016; 260:117-128 Regression of fibrosis/cirrhosis by Glycine propionyl-l-carnitine treatment in d-Galactosamine induced chronic liver damage.
Application : WB
Reactivity : Rat
Cheng YL et al. J. Mater. Chem. B. 2016; (38) Enhanced adhesion and differentiation of human mesenchymal stem cell inside apatite-mineralized/poly(dopamine)-coated poly(ε-caprolactone) scaffolds by stereolithography.
Wang Y et al. Sci Rep 2016; 6:24779 Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization.
Application : IHC
Reactivity : Pig
Kim TH et al. Tissue Eng Part A 2016; 22 (43289):698-706 Nanografted Substrata and Triculture of Human Pericytes, Fibroblasts, and Endothelial Cells for Studying the Effects on Angiogenesis.
Application : ICC/IF
Reactivity : Human
Lin C et al. J Cell Mol Med 2015; 19 (6):1346-56 Protease-activated receptor (PAR)-2 is required for PAR-1 signalling in pulmonary fibrosis.
Application : IHC-P
Reactivity : Mouse
Bao J et al. Sci Rep 2015; 5:10756 Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through * immobilization.
Application : IHC
Reactivity : Pig
Wu Q et al. Biomed Res Int 2015; 2015:785474 Optimizing perfusion-decellularization methods of porcine livers for clinical-scale whole-organ bioengineering.
Application : IHC
Reactivity : Pig
Delella FK et al. Life Sci 2012; 91 (25-26):1281-7 Fibrosis-related gene expression in the prostate is modulated by * treatment.
Application : IHC-Fr
Reactivity : Rat
Clone Reference
Sheehy EJ et al. PLoS One 2014; 9 (3):e90716 Altering the architecture of tissue engineered hypertrophic cartilaginous grafts facilitates vascularisation and accelerates mineralisation.
Application : IHC-P
Reactivity : Mouse
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399