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GPR171 antibody [N1], N-term

KO/KD
Anti-GPR171 antibody [N1], N-term used in Western Blot (WB). GTX108131

Cat. No. GTX108131

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GPR171 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
IHC Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:24043826).

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human GPR171. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036994

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor 171 , H963

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Orphan receptor.

Database

Research Area

DATA IMAGES

Anti-GPR171 antibody [N1], N-term used in Western Blot (WB). GTX108131

GTX108131 WB Image

KO/KD

The data was published in the journal Oncotarget in 2016.PMID: 26760963

REFERENCE

Application Reference

Akila Ram et al. Front Pain Res (Lausanne) 2021; 2 : 695396 GPR171 Agonist Reduces Chronic Neuropathic and Inflammatory Pain in Male, But Not Female Mice.
Application : IHC
Reactivity : Mouse
Dho SH et al. Oncotarget 2016; 7 (7) : 7856-65 GPR171 expression enhances proliferation and metastasis of lung cancer cells.
Wardman JH et al. Sci Signal 2016; 9 (430) : ra55 Identification of a small-molecule ligand that activates the neuropeptide receptor GPR171 and increases food intake.
Application : WB
Reactivity : Mouse
Wardman JH et al. Sci. Signal 2016; 9 (430) : ra55 RESEARCH ARTICLEPHARMACOLOGY Identification of a small-molecule ligand that activates the neuropeptide receptor GPR171 and increases food intake.
Application : WB, IHC-P
Reactivity : Mouse
Gomes I et al. Sci Signal 2016; 9 (425) : ra43 Identification of GPR83 as the receptor for the neuroendocrine peptide PEN.
Application : PLA
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169