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SEC13L1 antibody [N1C3]

Anti-SEC13L1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101055
Anti-SEC13L1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101055
Anti-SEC13L1 antibody [N1C3] used in Immunoprecipitation (IP). GTX101055
Anti-SEC13L1 antibody [N1C3] used in Western Blot (WB). GTX101055

Cat. No. GTX101055

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SEC13L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEC13L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951836

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SEC13 homolog, nuclear pore and COPII coat complex component , D3S1231E , SEC13L1 , SEC13R , npp-20

Background

The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SEC13L1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101055

GTX101055 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SEC13L1(GTX101055) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SEC13L1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101055

GTX101055 ICC/IF Image

SEC13L1 antibody [N1C3] detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SEC13L1 protein stained by SEC13L1 antibody [N1C3] (GTX101055) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SEC13L1 antibody [N1C3] used in Immunoprecipitation (IP). GTX101055

GTX101055 IP Image

SEC13L1 antibody immunoprecipitates SEC13L1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 μg of SEC13L1 antibody (GTX101055) 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody (GTX101055) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-SEC13L1 antibody [N1C3] used in Western Blot (WB). GTX101055

GTX101055 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC13L1 antibody [N1C3] (GTX101055) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lingyun Long et al. Nature 2021; 600 (7888) : 308-313 CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.
Application : WB, IP
Reactivity : Human
Wolfson RL et al. Nature 2017; 543 (7645) : 438-442 KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169