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Bassoon antibody [SAP7F407]

Anti-Bassoon antibody [SAP7F407] used in Immunohistochemistry (IHC). GTX13249
Anti-Bassoon antibody [SAP7F407] used in Western Blot (WB). GTX13249

Cat. No. GTX13249

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SAP7F407

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all Bassoon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP 2.5μg
IHC 1:400
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol, PBS, 0.1mM PMSF

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant rat bassoon fragment (residues 738-1035) expressed asa fusion protein in E.coli.

Purification

Protein G purified
This antibody has been affinity purified.

Conjugation

Unconjugated

RRID

AB_422275

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Bassoon (Presynaptic Cytomatrix Protein) , Znf231 , Bsn

Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors.Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.

Research Area

DATA IMAGES

Anti-Bassoon antibody [SAP7F407] used in Immunohistochemistry (IHC). GTX13249

GTX13249 IHC Image

Immunohistochemistry analysis of bassoon localization in the rat brain cerebellar molecular layer using Bassoon, mAb (SAP7F407)

Anti-Bassoon antibody [SAP7F407] used in Western Blot (WB). GTX13249

GTX13249 WB Image

Western blot analysis of rat brain tissue lysate using Bassoon antibody [SAP7F407].

REFERENCE

Application Reference

Garc?a-Arroyo R et al. Redox Biol 2023; 66 : 102862 Exacerbated response to oxidative stress in the Retinitis Pigmentosa Cerkl(KD/KO) mouse model triggers retinal degeneration pathways upon acute light stress.
R Garc?a-Arroyo et al. Redox Biology 2023; : 102862 Exacerbated response to oxidative stress in the Retinitis Pigmentosa CerklKD/KO mouse model triggers retinal degeneration pathways upon acute light stress
Application : IHC-Fr
Reactivity : Mouse
Ross JA et al. J Cell Sci 2017; 130 (10) : 1772-1784 Multiple roles of integrin-α3 at the neuromuscular junction.
Chen CY et al. Dev Neurobiol 2016; (Epub) Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Application : ICC/IF
Reactivity : Rat
Wentzel C et al. Neuron 2013; 78 (6) : 1012-23 mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking.
Application : ICC/IF
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399