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NOX1 antibody

Orthogonal
Anti-NOX1 antibody used in Western Blot (WB). GTX103888
Anti-NOX1 antibody used in Western Blot (WB). GTX103888
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888
Anti-NOX1 antibody used in Western Blot (WB). GTX103888
Anti-NOX1 antibody used in Western Blot (WB). GTX103888
Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103888
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888
Anti-NOX1 antibody used in Western Blot (WB). GTX103888

Cat. No. GTX103888

Reference 23

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NOX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

JC , BCL-1 , 293T , A431 , HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951012

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADPH oxidase 1 , GP91-2 , MOX1 , NOH-1 , NOH1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody (GTX103888) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody (GTX103888) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888

GTX103888 IHC-P Image

NOX1 antibody detects NOX1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
NOX1 antibody (GTX103888) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888

GTX103888 IHC-P Image

NOX1 antibody detects NOX1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NOX1 stained by NOX1 antibody (GTX103888) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

Sample (30 μg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX103888 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX103888 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103888

GTX103888 ICC/IF Image

NOX1 antibody detects NOX1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NOX1 protein stained by NOX1 antibody (GTX103888) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888

GTX103888 IHC-P Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2017.PMID: 29317986

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 29142252

REFERENCE

Application Reference

Mori M et al. Exp Anim 2023; 72 (2) : 233-241 Cyba and Nox2 mutant rats show different incidences of eosinophilia in the genetic background- and sex-dependent manner.
Qipeng Wu et al. Cell Death Dis 2022; 13 (4) : 341 Osteopontin promotes hepatocellular carcinoma progression through inducing JAK2/STAT3/NOX1-mediated ROS production.
Liu D et al. Cell Mol Gastroenterol Hepatol 2022; (Epub) Protein Kinase CK2 acts as a molecular brake to control NADPH oxidase 1 activation and colon inflammation.
Saurav Bhowmick et al. eNeuro 2021; 8 (4) Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.
Application : WB
Reactivity : Human
Hsiao-Ya Tsai et al. Front Immunol 2021; 12 : 740562 Pharmacological Activation Of Aldehyde Dehydrogenase 2 Protects Against Heatstroke-Induced Acute Lung Injury by Modulating Oxidative Stress and Endothelial Dysfunction.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Mori M et al. Exp Anim 2023; 72 (2):233-241 Cyba and Nox2 mutant rats show different incidences of eosinophilia in the genetic background- and sex-dependent manner.
Qipeng Wu et al. Cell Death Dis 2022; 13 (4):341 Osteopontin promotes hepatocellular carcinoma progression through inducing JAK2/STAT3/NOX1-mediated ROS production.
Liu D et al. Cell Mol Gastroenterol Hepatol 2022; (Epub) Protein Kinase CK2 acts as a molecular brake to control NADPH oxidase 1 activation and colon inflammation.
Yanhui Jiang et al. Front Oncol 2021; 11:629974 DDX19A Promotes Metastasis of Cervical Squamous Cell Carcinoma by Inducing NOX1-Mediated ROS Production.
Application : IHC-P
Reactivity : Human
Saurav Bhowmick et al. eNeuro 2021; 8 (4) Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.
Application : WB
Reactivity : Human
Hsiao-Ya Tsai et al. Front Immunol 2021; 12:740562 Pharmacological Activation Of Aldehyde Dehydrogenase 2 Protects Against Heatstroke-Induced Acute Lung Injury by Modulating Oxidative Stress and Endothelial Dysfunction.
Application : WB
Reactivity : Human
Cheng D et al. Mol Med Rep 2020; 22 (5):4151-4162 Protective effects of valsartan administration on doxorubicin?induced myocardial injury in rats and the role of oxidative stress and NOX2/NOX4 signaling.
Application : WB
Reactivity : Human
Finetti F et al. Cell Signal 2020; 68:109527 KRIT1 loss-mediated upregulation of NOX1 in stromal cells promotes paracrine pro-angiogenic responses.
Application : WB
Liang S et al. Gastroenterology 2019; 156 (4):1156-1172.e6 NADPH Oxidase 1 in Liver Macrophages Promotes Inflammation and Tumor Development in Mice.
Wang FT et al. Oncol Rep 2019; Upregulated NOX1 expression in gallbladder cancer?ssociated fibroblasts predicts a poor prognosis.
Cai YY et al. J Diabetes 2019; (Epub) Renoprotective effects of brown adipose tissue activation in diabetic mice.
Application : WB
Reactivity : Mouse
Feng YY et al. J Immunol 2019; 202 (9):2546-2557 Essential Role of NADPH Oxidase-Dependent Production of Reactive Oxygen Species in Maintenance of Sustained B Cell Receptor Signaling and B Cell Proliferation.
Application : WB
Reactivity : Mouse
Sandrini L et al. Oxid Med Cell Longev 2017; 2017:9258937 Apocynin Prevents Abnormal Megakaryopoiesis and Platelet Activation Induced by Chronic Stress.
Sandrini L et al. Oxidative Medicine and Cellular Longevity 2017; 2017:Article ID 9258937 Apocynin Prevents Abnormal Megakaryopoiesis and Platelet Activation Induced by Chronic Stress.
Application : IHC-P
Reactivity : Mouse
Jin L et al. Sci Rep 2017; 7 (1):15607 Gallic Acid Reduces Blood Pressure and Attenuates Oxidative Stress and Cardiac Hypertrophy in Spontaneously Hypertensive Rats.
Application : WB
Misawa H et al. Toxicol Appl Pharmacol 2017; 334:55-65 Prostacyclin mimetics afford protection against lipopolysaccharide/d-galactosamine-induced acute liver injury in mice.
Application : WB
Reactivity : Mouse
Lee M et al. Invest Ophthalmol Vis Sci 2016; 57 (4):1553-62 Interphotoreceptor Retinoid-Binding Protein Mitigates Cellular Oxidative Stress and Mitochondrial Dysfunction Induced by All-trans-Retinal.
Application : WB
Reactivity : Mouse
Qiu S et al. J Am Heart Assoc 2014; 3 (3) Increasing * Improves Vascular Function in Obese (db/db) Mice.
Application : WB
Wu JS et al. Mol Neurobiol 2013; 15-Deoxy-?12,14-PGJ 2, by activating peroxisome proliferator-activated receptor-gamma, suppresses p22phox transcription to protect brain endothelial cells against hypoxia-induced apoptosis.
Application : WB
Reactivity : Mouse
Dvoriantchikova G et al. Invest Ophthalmol Vis Sci 2012; 53 (6):2823-30 Neuronal NAD(P)H oxidases contribute to ROS production and mediate RGC death after ischemia.
Musset B et al. J Biol Chem 2012; 287 (12):9376-88 NOX5 in human spermatozoa: expression, function, and regulation.
Application : WB
Reactivity : Human
Wu CH et al. J Biol Chem 2012; 287 (30):25292-302 Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation.
Application : WB
Reactivity : Human
Mukohda M et al. Pharmacol Res 2012; 65 (1):33482 Long-term methylglyoxal treatment impairs smooth muscle contractility in organ-cultured rat mesenteric artery.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169