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14-3-3 (phospho Ser) antibody [3i2(4E2)]

Orthogonal
Anti-14-3-3 (phospho Ser) antibody [3i2(4E2)] used in Western Blot (WB). GTX14126

Cat. No. GTX14126

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3i2(4E2)

Isotype

 IgG1

Application

 WB, IP, ELISA

Reactivity

 Human, Zebrafish
Package
50 μl ($399)
See all 14-3-3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:4000
IP 1:20
ELISA 1:1000
Not tested in other applications.

Product Note

Recognizes peptides/proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. Weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences.

PROPERTIES

Form

Liquid

Buffer

10mM Sodium HEPES pH7.5, 150mM Sodium Chloride, 0.1mg/ml BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH).

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384336

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-14-3-3 (phospho Ser) antibody [3i2(4E2)] used in Western Blot (WB). GTX14126

GTX14126 WB Image

Orthogonal

WB analysis of calyculin A treated or untreated A431 cells using GTX14126 14-3-3 (phospho Ser) antibody [3i2(4E2)].

REFERENCE

Application Reference

Kumar D et al. Sci Rep 2018; 8 (1) : 4547 Microvillar and ciliary defects in zebrafish lacking an actin-binding bioactive peptide amidating enzyme.
Application : WB
Reactivity : Zebrafish
Submit a Reference

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399