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NPAS1 antibody

Anti-NPAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105876
Anti-NPAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105876
Anti-NPAS1 antibody used in Western Blot (WB). GTX105876
Anti-NPAS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105876
Anti-NPAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105876
Anti-NPAS1 antibody used in Immunohistochemistry (IHC). GTX105876

Cat. No. GTX105876

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NPAS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

U87-MG , IMR32 , SK-N-AS

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NPAS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885261

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neuronal PAS domain protein 1 , MOP5 , PASD5 , bHLHe11

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. Studies of a related mouse gene suggest that it functions in neurons. The exact function of this gene is unclear, but it may play protective or modulatory roles during late embryogenesis and postnatal development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NPAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105876

GTX105876 IHC-P Image

NPAS1 antibody detects NPAS1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
NPAS1 antibody (GTX105876) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NPAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105876

GTX105876 IHC-P Image

NPAS1 antibody detects NPAS1 protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
NPAS1 antibody (GTX105876) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NPAS1 antibody used in Western Blot (WB). GTX105876

GTX105876 WB Image

NPAS1 antibody detects NPAS1 protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg IMR32 whole cell lysate/extract
C. 30 μg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
NPAS1 antibody (GTX105876) dilution: 1:1000

Anti-NPAS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105876

GTX105876 ICC/IF Image

NPAS1 antibody detects NPAS1 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: NPAS1 protein stained by NPAS1 antibody (GTX105876) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NPAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105876

GTX105876 IHC-P Image

NPAS1 antibody detects NPAS1 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
NPAS1 antibody (GTX105876) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NPAS1 antibody used in Immunohistochemistry (IHC). GTX105876

GTX105876 IHC Image

The data was published in the journal Elife in 2018.PMID: 29714166

REFERENCE

Application Reference

Klug JR et al. Elife 2018; 7 Differential inputs to striatal cholinergic and parvalbumin interneurons imply functional distinctions.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169