alpha Synuclein (phospho Ser129) antibody

Cat. No. GTX54991

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all alpha Synuclein products

View product citations for antibody GTX54991 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
ICC/IF	1:100 - 1:500
IHC-P	1:100 - 1:200
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of alpha Synuclein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887909

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm,Membrane,Nucleus,Cell junction, synapse,Secreted

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]

Database

Gene ID: 6622 SNCA
UniProt: P37840 SNCA

Research Area

DATA IMAGES

GTX54991 ICC/IF Image

ICC/IF analysis of formalin-fixed HEK293T cells using GTX54991 alpha Synuclein (phospho Ser129) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX54991 WB Image

WB analysis of HEK293T (A), mouse brain (B) whole cell lysates using GTX54991 alpha Synuclein (phospho Ser129) antibody.

GTX54991 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX54991 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)