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Nkx2.5 antibody

Anti-Nkx2.5 antibody used in IHC (Whole mount) (IHC-Wm). GTX128357

Cat. No. GTX128357

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Nkx2.5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Wm 1:50-1:500
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Nkx2.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885763

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NK2 homeobox 5 , nk2.5 , nkx2-5 , sb:eu581 , zgc:111912

Database

Research Area

DATA IMAGES

Anti-Nkx2.5 antibody used in IHC (Whole mount) (IHC-Wm). GTX128357

GTX128357 IHC-Wm Image

Nkx2.5 antibody detects Nkx2.5 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Nkx2.5 antibody (GTX128357) dilution: 1:50.

REFERENCE

Application Reference

Kula-Alwar D et al. Dev Biol 2021; 470 : 95-107 Mef2c factors are required for early but not late addition of cardiomyocytes to the ventricle.
Ren J et al. Dev Cell 2019; Canonical Wnt5b Signaling Directs Outlying Nkx2.5+ Mesoderm into Pacemaker Cardiomyocytes.
Application : IHC-Wm
Reactivity : Zebrafish
Song YC et al. PLoS Genet 2019; 15 (5) : e1008165 HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development.
Application : IHC-Wm
Reactivity : Zebrafish
Dohn TE et al. PLoS Genet 2019; 15 (2) : e1007962 Nr2f-dependent allocation of ventricular cardiomyocyte and pharyngeal muscle progenitors.
Application : IHC-Wm
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169