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RCC2 antibody [N3C3]

Anti-RCC2 antibody [N3C3] used in Western Blot (WB). GTX107094
Anti-RCC2 antibody [N3C3] used in Western Blot (WB). GTX107094
Anti-RCC2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107094

Cat. No. GTX107094

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RCC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Mouse brain , H1299 , Molt-4

Predict Reactivity

Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RCC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951673

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of chromosome condensation 2 , TD-60

Cellular Localization

Nucleus , nucleolus , Centromere , Spindle

Background

Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.

Database

Research Area

DATA IMAGES

Anti-RCC2 antibody [N3C3] used in Western Blot (WB). GTX107094

GTX107094 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX107094 diluted at 1:1000

Anti-RCC2 antibody [N3C3] used in Western Blot (WB). GTX107094

GTX107094 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX107094 diluted at 1:1000

Anti-RCC2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107094

GTX107094 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using RCC2(GTX107094) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169