CD97/ADGRE5 is a member of the “Adhesion G protein-coupled receptor (GPCR)” subfamily of the GPCR superfamily. It is expressed on the surface of T cells, monocytes, neutrophils, epithelial cells, muscle cells, and various cancer cells. It interacts with multiple ligands, including DAF/CD55, and has roles in innate immunity, T cell activation, granulocyte migration, autoimmune disease, and tumor initiation and progression (1). Recent studies also indicate that CD97 may be involved in mechano-transduction in certain adherent cell types (1).

GeneTex is proud to offer a newly launched recombinant rabbit monoclonal CD97 antibody [HL1925] (GTX637674) to detect CD97/ADGRE5 in many applications. This antibody also detects both the glycosylated and deglycosylated forms of CD97 by western blot. Please see the highlighted images below and visit www.genetex.com for more details.

 

CD97 antibody [HL1925] (GTX637674)

Detection of glycosylated and deglycosylated CD97 proteins in Mock or Tunicamycin-treated U937 cell lysates.	Detection of CD97 in boiled and unboiled U937 whole cell lysate (WCE) or membrane extract (ME).	Detection of overexpressed CD97 in transfected 293T cell lysates.	Immunoprecipitation (IP) of CD97 protein from U937 cell lysates.

 

Flow cytometry analysis of U937 cells stained with secondary antibody only (GTX213110-04) (Blue) or CD97 antibody [HL1925] (GTX637674) (Red).	Detection of CD97 in paraffin-embedded mouse colon tissue.

 

Reference:

1. Pharmaceuticals (Basel) . 2022 Feb 11;15(2):219. doi: 10.3390/ph15020219.