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12S globulin antibody

Anti-12S globulin antibody used in Western Blot (WB). GTX00936

Cat. No. GTX00936

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, EM, IHC

Reactivity

 Arabidopsis thaliana
Package
200 μg ($419)
See all 12S globulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:3000-1:10000
ELISA Assay dependent
EM 1:50
IHC 1:100
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Product Note

It reacts with precursor and alpha-subunit of Arabidopsis thaliana 12S globulin.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Arabidopsis thaliana

Immunogen

A. thaliana 12S globulin alpha-subunit purified on SDS-PAGE

Purification

Protein A/G purified
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Protein storage vacuole

Background

Encodes a 12S seed storage protein that is tyrosine-phosphorylated and its phosphorylation state is modulated in response to ABA in Arabidopsis thaliana seeds.

Database

Research Area

DATA IMAGES

Anti-12S globulin antibody used in Western Blot (WB). GTX00936

GTX00936 WB Image

WB analysis of Arabidopsis dry seeds extract using GTX00936 12S globulin antibody. P12S is the precursor of 12S globulin.
Lane 1 : Wild-type seed
Lane 2 : Atvst1-1 mutant (defective in storage protein transport)
Dilution : 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 419