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14-3-3 alpha/beta antibody [SD0837]

Recombinant antibody
Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978
Anti-14-3-3 alpha/beta antibody [SD0837] used in Flow cytometry (FACS). GTX00978
Anti-14-3-3 alpha/beta antibody [SD0837] used in Western Blot (WB). GTX00978
Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978
Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978
Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978
Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978
Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978
Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978

Cat No. GTX00978

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 SD0837

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta,GW128,HELS1,HS1,KCIP1,YWHAA,YWHAB,tyrosine 3monooxygenase/tryptophan 5monooxygenase activation protein beta,14-3-3 beta,14-3-3 beta/alpha,14-3-3 alpha

Cellular Localization

Cytoplasm,Melanosome

Background

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978

GTX00978 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-14-3-3 alpha/beta antibody [SD0837] used in Flow cytometry (FACS). GTX00978

GTX00978 FACS Image

FACS analysis of HeLa cells using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-14-3-3 alpha/beta antibody [SD0837] used in Western Blot (WB). GTX00978

GTX00978 WB Image

WB analysis of various samples using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Dilution : 1:1000

Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978

GTX00978 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978

GTX00978 IHC-P Image

IHC-P analysis of mouse skin tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].

Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978

GTX00978 ICC/IF Image

ICC/IF analysis of PFA-fixed SHG-44 cells using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978

GTX00978 IHC-P Image

IHC-P analysis of human breast tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].

Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978

GTX00978 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].

Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978

GTX00978 IHC-P Image

IHC-P analysis of human breast carcinoma tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].

REFERENCE

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REVIEW

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Package List Price ($)
$ 369