APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:5000 |
| 1:100-1:500 |
| 1:50-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta , GW128 , HELS1 , HS1 , KCIP1 , YWHAA , YWHAB , tyrosine 3monooxygenase/tryptophan 5monooxygenase activation protein beta , 14-3-3 beta , 14-3-3 beta/alpha , 14-3-3 alpha
Cellular Localization
Cytoplasm,Melanosome
Background
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_ICC-IF_9_20030421_349.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
|
GTX00978 ICC/IF Image
ICC/IF analysis of PFA-fixed A431 cells using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
|
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Flow cytometry (FACS). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_FACS_15_20030421_540.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Flow cytometry (FACS). GTX00978")
|
GTX00978 FACS Image
FACS analysis of HeLa cells using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50
|
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Western Blot (WB). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_WB_7_20030421_777.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Western Blot (WB). GTX00978")
|
GTX00978 WB Image
WB analysis of various samples using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Dilution : 1:1000
|
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_ICC-IF_8_20030421_548.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
|
GTX00978 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
|
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_IHC-P_13_20030421_668.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
|
GTX00978 IHC-P Image
IHC-P analysis of mouse skin tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].
|
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_ICC-IF_10_20030421_396.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
|
GTX00978 ICC/IF Image
ICC/IF analysis of PFA-fixed SHG-44 cells using GTX00978 14-3-3 alpha/beta antibody [SD0837].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
|
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_IHC-P_14_20030421_308.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
|
GTX00978 IHC-P Image
IHC-P analysis of human breast tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].
|
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_IHC-P_12_20030421_834.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
|
GTX00978 IHC-P Image
IHC-P analysis of mouse brain tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].
|
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/middle/GTX00978/GTX00978_20200303_IHC-P_11_20030421_145.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
|
GTX00978 IHC-P Image
IHC-P analysis of human breast carcinoma tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].
|
REFERENCE
There are currently no references for 14-3-3 alpha/beta antibody [SD0837] (GTX00978). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for 14-3-3 alpha/beta antibody [SD0837] (GTX00978). Be the first to share your experience with this product.
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_ICC-IF_9_20030421_349.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Flow cytometry (FACS). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_FACS_15_20030421_540.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Flow cytometry (FACS). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Western Blot (WB). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_WB_7_20030421_777.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Western Blot (WB). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_ICC-IF_8_20030421_548.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_IHC-P_13_20030421_668.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_ICC-IF_10_20030421_396.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_IHC-P_14_20030421_308.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_IHC-P_12_20030421_834.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/small/GTX00978/GTX00978_20200303_IHC-P_11_20030421_145.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_ICC-IF_9_20030421_349.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Flow cytometry (FACS). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_FACS_15_20030421_540.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Flow cytometry (FACS). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Western Blot (WB). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_WB_7_20030421_777.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Western Blot (WB). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_ICC-IF_8_20030421_548.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_IHC-P_13_20030421_668.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_ICC-IF_10_20030421_396.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_IHC-P_14_20030421_308.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_IHC-P_12_20030421_834.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
![Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978](/upload/website/prouct_img/normal/GTX00978/GTX00978_20200303_IHC-P_11_20030421_145.jpg "Anti-14-3-3 alpha/beta antibody [SD0837] used in IHC (Paraffin sections) (IHC-P). GTX00978")
![Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419](/upload/website/prouct_img/small/GTX627419/GTX627419_40878_IP_18121411_117.jpg "Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419")
![Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567](/upload/website/prouct_img/small/GTX100567/GTX100567_39471_WB_B_19011506_797.jpg "Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567")
