APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:1000-1:5000 |
1:100-1:500 |
1:50-1:200 |
1:50-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta , GW128 , HELS1 , HS1 , KCIP1 , YWHAA , YWHAB , tyrosine 3monooxygenase/tryptophan 5monooxygenase activation protein beta , 14-3-3 beta , 14-3-3 beta/alpha , 14-3-3 alpha
Cellular Localization
Cytoplasm,Melanosome
Background
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00978 ICC/IF Image
ICC/IF analysis of PFA-fixed A431 cells using GTX00978 14-3-3 alpha/beta antibody [SD0837]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS
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GTX00978 FACS Image
FACS analysis of HeLa cells using GTX00978 14-3-3 alpha/beta antibody [SD0837]. Red : primary antibody Black : unlabelled control Dilution : 1:50
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GTX00978 WB Image
WB analysis of various samples using GTX00978 14-3-3 alpha/beta antibody [SD0837]. Lane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : HepG2 cell lysate Dilution : 1:1000
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GTX00978 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX00978 14-3-3 alpha/beta antibody [SD0837]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS
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GTX00978 IHC-P Image
IHC-P analysis of mouse skin tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].
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GTX00978 ICC/IF Image
ICC/IF analysis of PFA-fixed SHG-44 cells using GTX00978 14-3-3 alpha/beta antibody [SD0837]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS
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GTX00978 IHC-P Image
IHC-P analysis of human breast tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].
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GTX00978 IHC-P Image
IHC-P analysis of mouse brain tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].
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GTX00978 IHC-P Image
IHC-P analysis of human breast carcinoma tissue section using GTX00978 14-3-3 alpha/beta antibody [SD0837].
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REFERENCE
There are currently no references for 14-3-3 alpha/beta antibody [SD0837] (GTX00978). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for 14-3-3 alpha/beta antibody [SD0837] (GTX00978). Be the first to share your experience with this product.