PRODUCT
Summary
14-3-3 antibody detects 14-3-3 proteins, which in mammals exist in seven isoforms (β, γ, ε, η, ζ, σ, and τ/θ) and have predicted molecular weights of ~28 kDa. 14-3-3 proteins are homo- or heterodimeric adaptors involved in the regulation of various signaling pathways such as mTOR, AMPK, and MAPK.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.19 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human 14-3-3 beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887086
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GW128 antibody , HS1 antibody , KCIP1 antibody , YWHAA antibody , YWHAB antibody , tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta antibody
Cellular Localization
Cytoplasm , Melanosome
Background
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]
Research Area
DATA IMAGES
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GTX133736 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 antibody (GTX133736) diluted at 1:3000 and competitor's antibody (sc-629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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REFERENCE
There are currently no references for 14-3-3 antibody (GTX133736). Be the first to share your publications with this product.
REVIEW
14-3-3 antibody
Cat. No. GTX133736
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 12-Jan-2018
Application Tested :
WB
Sample Species :
Ms
Sample :
C2C12 cells
Amount used :
40 μg
Blocking :
1% BSA in TBS, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
