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14-3-3 antibody

Anti-14-3-3 antibody used in Western Blot (WB). GTX133736

Cat No. GTX133736

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

14-3-3 antibody detects 14-3-3 proteins, which in mammals exist in seven isoforms (β, γ, ε, η, ζ, σ, and τ/θ) and have predicted molecular weights of ~28 kDa. 14-3-3 proteins are homo- or heterodimeric adaptors involved in the regulation of various signaling pathways such as mTOR, AMPK, and MAPK.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human 14-3-3 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887086

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GW128 antibody , HS1 antibody , KCIP1 antibody , YWHAA antibody , YWHAB antibody , tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta antibody

Cellular Localization

Cytoplasm , Melanosome

Background

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-14-3-3 antibody used in Western Blot (WB). GTX133736

GTX133736 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 antibody (GTX133736) diluted at 1:3000 and competitor's antibody (sc-629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

There are currently no references for 14-3-3 antibody (GTX133736). Be the first to share your publications with this product.

REVIEW

14-3-3 antibody Cat No. GTX133736

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 12-Jan-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : C2C12 cells
Amount used : 40 μg
Blocking : 1% BSA in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf