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14-3-3 beta antibody [4E1]

Anti-14-3-3 beta antibody [4E1] used in Western Blot (WB). GTX12341

Cat. No. GTX12341

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E1

Isotype

 IgG2b

Application

 WB, IP

Reactivity

 Mouse, Rat
Package
50 μg ($399)
See all 14-3-3 beta products

APPLICATION

Application Note

IP: Use at a concentration of 5 - 10 μg/ml. WB: Use at a concentration of 1 - 5 μg/ml. Detects a band of approximately 29 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brain tissue extract.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 10mg/ml Sucrose

Preservative

1mg/ml Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant full length protein (Rat).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta , GW128 , HEL-S-1 , HS1 , KCIP-1 , YWHAA

Cellular Localization

Cytoplasm

Background

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-14-3-3 beta antibody [4E1] used in Western Blot (WB). GTX12341

GTX12341 WB Image

Western blot analysis of mouse brain tissue extract, probed with GTX12341
Lane 1: Mouse IgG
Lane 2: Anti-14-3-3β

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399