GeneTex
United States (US)

14-3-3 beta antibody [4E1]

14-3-3-beta-antibody-4E1_WB_GTX12341-1_18121409_709.jpg

Cat No. GTX12341

Host Mouse
Clonality Monoclonal
Clone Name 4E1
Isotype IgG2b
Application WB, IP
Reactivity Mouse, Rat
APPLICATION

Application Note

IP: Use at a concentration of 5 - 10 μg/ml. WB: Use at a concentration of 1 - 5 μg/ml. Detects a band of approximately 29 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brain tissue extract.
PROPERTIES

Form

Liquid

Buffer

Preservative: 1mg/ml Sodium Azide; Constituents: PBS, 10mg/ml Sucrose. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant full length protein (Rat).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta,Gw128,Hel-S-1,Hs1,Kcip-1,Ywhaa,Ywhab

Cellular Localization

Cytoplasmic

Background

14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasma of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

Database

Research Area

DATA IMAGES
14-3-3-beta-antibody-4E1_WB_GTX12341-1_18121409_709.jpg

GTX12341 WB Image

Western blot analysis of mouse brain tissue extract, probed with GTX12341
Lane 1: Mouse IgG
Lane 2: Anti-14-3-3β

Package List Price ($)
$ 319