APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , 293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human 14-3-3 beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952625
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta , GW128 , HEL-S-1 , HS1 , KCIP-1 , YWHAA
Cellular Localization
Cytoplasm , Melanosome
Background
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296](/templates/default/js/lightbox/images/loading.gif "Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296")
|
GTX113296 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C2] (GTX113296) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX113296 IHC-P Image
14-3-3 beta antibody [N1C2] detects 14-3-3 beta protein at cytoplasm in rat cervix by immunohistochemical analysis.
Sample: Paraffin-embedded rat cervix.
14-3-3 beta antibody [N1C2] (GTX113296) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113296 WB Image
Sample (20 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113296 diluted at 1:10000
|
|
|
GTX113296 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using 14-3-3 beta(GTX113296) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113296 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (GTX113296) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
|
|
|
GTX113296 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (GTX113296) antibody at 1:200 dilution.
|
REFERENCE
There are currently no references for 14-3-3 beta antibody [N1C2] (GTX113296). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for 14-3-3 beta antibody [N1C2] (GTX113296). Be the first to share your experience with this product.
![Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296](/upload/website/prouct_img/small/GTX113296/GTX113296_40114_20181221_WB_shRNA_watermark_w_23060501_611.webp "Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113296](/upload/website/prouct_img/small/GTX113296/GTX113296_40114_20150601_IHC_R_w_23060501_862.webp "Anti-14-3-3 beta antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296](/upload/website/prouct_img/small/GTX113296/GTX113296_40114_WB_M_Brain_w_23060501_761.webp "Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113296](/upload/website/prouct_img/small/GTX113296/GTX113296_40114_IHC_w_23060501_148.webp "Anti-14-3-3 beta antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296](/upload/website/prouct_img/small/GTX113296/GTX113296_40114_20170804_WB_competitor_watermark_w_23060501_390.webp "Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113296](/upload/website/prouct_img/small/GTX113296/GTX113296_IFA_w_23060501_850.webp "Anti-14-3-3 beta antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296](/upload/website/prouct_img/normal/GTX113296/GTX113296_40114_20181221_WB_shRNA_watermark_w_23060501_611.webp "Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113296](/upload/website/prouct_img/normal/GTX113296/GTX113296_40114_20150601_IHC_R_w_23060501_862.webp "Anti-14-3-3 beta antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296](/upload/website/prouct_img/normal/GTX113296/GTX113296_40114_WB_M_Brain_w_23060501_761.webp "Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113296](/upload/website/prouct_img/normal/GTX113296/GTX113296_40114_IHC_w_23060501_148.webp "Anti-14-3-3 beta antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296](/upload/website/prouct_img/normal/GTX113296/GTX113296_40114_20170804_WB_competitor_watermark_w_23060501_390.webp "Anti-14-3-3 beta antibody [N1C2] used in Western Blot (WB). GTX113296")
![Anti-14-3-3 beta antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113296](/upload/website/prouct_img/normal/GTX113296/GTX113296_IFA_w_23060501_850.webp "Anti-14-3-3 beta antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113296")