GeneTex
United States (US)

14-3-3 beta + zeta antibody [22-IID8B]

14-3-3-beta-zeta-antibody-22-IID8B_WB_GTX12347-1_18121409_783.jpg

Cat No. GTX12347

Host Mouse
Clonality Monoclonal
Clone Name 22-IID8B
Isotype IgG
Application WB, ICC/IF, IHC
Reactivity Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Pig, Monkey
APPLICATION

Application Note

ICC: Use at an assay dependent dilution. IHC: Use at a dilution of 1/50. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 29 and 30 kDa. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

HeLa cell lysate
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Lysed transformed amnion cells (AMA) (Human).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

YWHAA, KCIP1, 1433 beta + zeta, HS1, GW128, 14-3-3-zeta, KCIP-1, MGC111427, MGC126532, MGC138156, YWHAD, KCIP 1, 14 3 3 zeta, 1433zeta

Cellular Localization

Cytoplasmic

Background

14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

Research Area

DATA IMAGES
14-3-3-beta-zeta-antibody-22-IID8B_WB_GTX12347-1_18121409_783.jpg

GTX12347 WB Image

Western blot analysis of human epidermoid carcinoma A431 cell lysate, probed with GTX12347

Package List Price ($)
$ 319