APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing result, this antibody does not cross react with human 14-3-3 zeta, human 14-3-3 beta, human 14-3-3 eta, human 14-3-3 gamma, human 14-3-3 sigma, and human 14-3-3 tau protein.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT4F8 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-5 epsiilon protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , HEL2 , KCIP1 , MDCR , MDS , YWHAE , tyrosine 3monooxygenase/tryptophan 5monooxygenase activation protein epsilon , 14-3-3 epsilon , 1433E
Cellular Localization
Nucleus,Cytoplasm,Melanosome
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]
Database
Research Area
DATA IMAGES
![Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563](/templates/default/js/lightbox/images/loading.gif "Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563")
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GTX57563 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57563 14-3-3 epsilon antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57563 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57563 14-3-3 epsilon antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57563 WB Image
WB analysis of various samples using GTX57563 14-3-3 epsilon antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : Mouse Brain tissue lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57563 WB Image
WB analysis of various samples using GTX57563 14-3-3 epsilon antibody.
Lane 1 : Recombinant Human 14-3-3 zeta protein
Lane 2 : Recombinant Human 14-3-3 beta protein
Lane 3 : Recombinant Human 14-3-3 epsilon protein
Lane 4 : Recombinant Human 14-3-3 eta protein
Lane 5 : Recombinant Human 14-3-3 gamma protein
Lane 6 : Recombinant Human 14-3-3 sigma protein
Lane 7 : Recombinant Human 14-3-3 tau protein
Loading : 50 ng
Dilution : 1:1000
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REFERENCE
There are currently no references for 14-3-3 epsilon antibody [AT4F8] (GTX57563). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for 14-3-3 epsilon antibody [AT4F8] (GTX57563). Be the first to share your experience with this product.
![Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563](/upload/website/prouct_img/small/GTX57563/GTX57563_20200204_IF1_w_23061123_927.webp "Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563")
![Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563](/upload/website/prouct_img/small/GTX57563/GTX57563_20200204_IF2_w_23061123_294.webp "Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563")
![Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563](/upload/website/prouct_img/small/GTX57563/GTX57563_20200204_WB1_w_23061123_751.webp "Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563")
![Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563](/upload/website/prouct_img/small/GTX57563/GTX57563_20200204_WB2_w_23061123_601.webp "Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563")
![Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563](/upload/website/prouct_img/normal/GTX57563/GTX57563_20200204_IF1_w_23061123_927.webp "Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563")
![Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563](/upload/website/prouct_img/normal/GTX57563/GTX57563_20200204_IF2_w_23061123_294.webp "Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563")
![Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563](/upload/website/prouct_img/normal/GTX57563/GTX57563_20200204_WB1_w_23061123_751.webp "Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563")
![Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563](/upload/website/prouct_img/normal/GTX57563/GTX57563_20200204_WB2_w_23061123_601.webp "Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563")