GeneTex
  • Country / Location Selection

United States (US)

14-3-3 epsilon antibody [AT4F8]

Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563
Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563
Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563
Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563

Cat. No. GTX57563

Host

Mouse

Clonality

Monoclonal

Clone Name

AT4F8

Isotype

IgG2b

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Product Note

Based on internal testing result, this antibody does not cross react with human 14-3-3 zeta, human 14-3-3 beta, human 14-3-3 eta, human 14-3-3 gamma, human 14-3-3 sigma, and human 14-3-3 tau protein.

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.4), 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT4F8 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-5 epsiilon protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , HEL2 , KCIP1 , MDCR , MDS , YWHAE , tyrosine 3monooxygenase/tryptophan 5monooxygenase activation protein epsilon , 14-3-3 epsilon , 1433E

Cellular Localization

Nucleus,Cytoplasm,Melanosome

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES

Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563

GTX57563 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57563 14-3-3 epsilon antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-14-3-3 epsilon antibody [AT4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57563

GTX57563 ICC/IF Image

ICC/IF analysis of A549 cells using GTX57563 14-3-3 epsilon antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563

GTX57563 WB Image

WB analysis of various samples using GTX57563 14-3-3 epsilon antibody.
Lane 1 : Recombinant Human 14-3-3 zeta protein
Lane 2 : Recombinant Human 14-3-3 beta protein
Lane 3 : Recombinant Human 14-3-3 epsilon protein
Lane 4 : Recombinant Human 14-3-3 eta protein
Lane 5 : Recombinant Human 14-3-3 gamma protein
Lane 6 : Recombinant Human 14-3-3 sigma protein
Lane 7 : Recombinant Human 14-3-3 tau protein
Loading : 50 ng
Dilution : 1:1000

Anti-14-3-3 epsilon antibody [AT4F8] used in Western Blot (WB). GTX57563

GTX57563 WB Image

WB analysis of various samples using GTX57563 14-3-3 epsilon antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : Mouse Brain tissue lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

There are currently no references for 14-3-3 epsilon antibody [AT4F8] (GTX57563). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for 14-3-3 epsilon antibody [AT4F8] (GTX57563). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399