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14-3-3 epsilon antibody [N1C3]

Anti-14-3-3 epsilon antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109090
Anti-14-3-3 epsilon antibody [N1C3] used in Immunoprecipitation (IP). GTX109090
Anti-14-3-3 epsilon antibody [N1C3] used in Western Blot (WB). GTX109090
Anti-14-3-3 epsilon antibody [N1C3] used in Western Blot (WB). GTX109090
Anti-14-3-3 epsilon antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109090

Cat. No. GTX109090

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all 14-3-3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human 14-3-3 epsilon Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952629

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , 14-3-3E , HEL2 , KCIP-1 , MDCR , MDS

Cellular Localization

Cytoplasm , Melanosome

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-14-3-3 epsilon antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109090

GTX109090 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon(GTX109090) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 epsilon antibody [N1C3] used in Immunoprecipitation (IP). GTX109090

GTX109090 IP Image

14-3-3 epsilon antibody [N1C3] immunoprecipitates YWHAE protein in IP experiments.
IP samples: HeLa whole cell extract
A. 40 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of YWHAE protein by 4 μg 14-3-3 epsilon antibody [N1C3] (GTX109090)
12 % SDS-PAGE
The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] (GTX109090) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-14-3-3 epsilon antibody [N1C3] used in Western Blot (WB). GTX109090

GTX109090 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] (GTX109090) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 epsilon antibody [N1C3] used in Western Blot (WB). GTX109090

GTX109090 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] (GTX109090) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 epsilon antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109090

GTX109090 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 epsilon(GTX109090) antibody at 1:200 dilution.

REFERENCE

Application Reference

Iwahori S et al. J Biol Chem 2022; 298 (11) : 102513 Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability.
Application : WB, ICC/IF, IP
Reactivity : Human
Yang YF et al. Kaohsiung J Med Sci 2019; (Epub) YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells.
Application : WB, IHC-P
Reactivity : Human
Brandi J et al. J Proteomics 2016; 136 : 43112 Secretome protein signature of human pancreatic cancer stem-like cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169