GeneTex

  • Country / Location Selection

United States (US)

14-3-3 epsilon antibody [N2C3]

Anti-14-3-3 epsilon antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113576
Anti-14-3-3 epsilon antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113576
Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576
Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576

Cat. No. GTX113576

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all 14-3-3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Mouse brain , H1299

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 epsilon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036123

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , 14-3-3E , HEL2 , KCIP-1 , MDCR , MDS

Cellular Localization

Cytoplasm , Melanosome

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-14-3-3 epsilon antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113576

GTX113576 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB231 xenograft, using 14-3-3 epsilon(GTX113576) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 epsilon antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113576

GTX113576 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using 14-3-3 epsilon(GTX113576) antibody at 1:200 dilution.

Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576

GTX113576 WB Image

Sample (20 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113576 diluted at 1:10000

Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576

GTX113576 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX113576 diluted at 1:1000

REFERENCE

There are currently no references for 14-3-3 epsilon antibody [N2C3] (GTX113576). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for 14-3-3 epsilon antibody [N2C3] (GTX113576). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169