APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , H1299
Predict Reactivity
Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.52 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human 14-3-3 epsilon. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036123
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , 14-3-3E , HEL2 , KCIP-1 , MDCR , MDS
Cellular Localization
Cytoplasm , Melanosome
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576](/templates/default/js/lightbox/images/loading.gif "Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576")
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GTX113576 WB Image
Sample (20 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113576 diluted at 1:10000
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GTX113576 IHC-P Image
Immunohistochemical analysis of paraffin-embedded MDA-MB231 xenograft, using 14-3-3 epsilon(GTX113576) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113576 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX113576 diluted at 1:1000
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GTX113576 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using 14-3-3 epsilon(GTX113576) antibody at 1:200 dilution.
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REFERENCE
There are currently no references for 14-3-3 epsilon antibody [N2C3] (GTX113576). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for 14-3-3 epsilon antibody [N2C3] (GTX113576). Be the first to share your experience with this product.
![Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576](/upload/website/prouct_img/small/GTX113576/GTX113576_40142_WB_M_Brain_18121410_530.jpg "Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576")
![Anti-14-3-3 epsilon antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113576](/upload/website/prouct_img/small/GTX113576/GTX113576_40142_IHC_18121410_598.jpg "Anti-14-3-3 epsilon antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113576")
![Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576](/upload/website/prouct_img/small/GTX113576/GTX113576_WB_18121410_517.jpg "Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576")
![Anti-14-3-3 epsilon antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113576](/upload/website/prouct_img/small/GTX113576/GTX113576_40142_IFA_18121410_867.jpg "Anti-14-3-3 epsilon antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113576")
![Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576](/upload/website/prouct_img/normal/GTX113576/GTX113576_40142_WB_M_Brain_18121410_530.jpg "Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576")
![Anti-14-3-3 epsilon antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113576](/upload/website/prouct_img/normal/GTX113576/GTX113576_40142_IHC_18121410_598.jpg "Anti-14-3-3 epsilon antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113576")
![Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576](/upload/website/prouct_img/normal/GTX113576/GTX113576_WB_18121410_517.jpg "Anti-14-3-3 epsilon antibody [N2C3] used in Western Blot (WB). GTX113576")
![Anti-14-3-3 epsilon antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113576](/upload/website/prouct_img/normal/GTX113576/GTX113576_40142_IFA_18121410_867.jpg "Anti-14-3-3 epsilon antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113576")