APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:300-1:10000 |
| 1-4 μl/mg of lysate |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.2μm-filtered PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant Human 14-3-3 epsilon / YWHAE protein
Purification
Purified by Protein A and Antigen Affinity
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , 14-3-3E , HEL2 , KCIP-1 , MDCR , MDS
Cellular Localization
Nucleus , Cytoplasm
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]
Database
Research Area
DATA IMAGES
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GTX02298 IP Image
IP analysis of Jurkat whole cell lysate using GTX02298 14-3-3 epsilon antibody.
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
50 % Protein G agarose : 15 μl
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GTX02298 WB Image
WB analysis of various samples using GTX02298 14-3-3 epsilon antibody.
Lane A : HepG2 whole cell lysate
Lane B : Jurkat whole cell lysate
Lane C : SH-SY-5Y whole cell lysate
Lane D : SW480 whole cell lysate
Dilution : 1:500
Loading : 30 μg
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GTX02298 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX02298 14-3-3 epsilon antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
Permeabilzation : 0.3% Triton X-100
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REFERENCE
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REVIEW
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