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14-3-3 epsilon antibody

Anti-14-3-3 epsilon antibody used in Immunoprecipitation (IP). GTX02298
Anti-14-3-3 epsilon antibody used in Western Blot (WB). GTX02298
Anti-14-3-3 epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02298

Cat. No. GTX02298

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:300-1:10000
IP 1-4 μl/mg of lysate
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human 14-3-3 epsilon / YWHAE protein

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , 14-3-3E , HEL2 , KCIP-1 , MDCR , MDS

Cellular Localization

Nucleus , Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES

Anti-14-3-3 epsilon antibody used in Immunoprecipitation (IP). GTX02298

GTX02298 IP Image

IP analysis of Jurkat whole cell lysate using GTX02298 14-3-3 epsilon antibody.
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
50 % Protein G agarose : 15 μl

Anti-14-3-3 epsilon antibody used in Western Blot (WB). GTX02298

GTX02298 WB Image

WB analysis of various samples using GTX02298 14-3-3 epsilon antibody.
Lane A : HepG2 whole cell lysate
Lane B : Jurkat whole cell lysate
Lane C : SH-SY-5Y whole cell lysate
Lane D : SW480 whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-14-3-3 epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02298

GTX02298 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02298 14-3-3 epsilon antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
Permeabilzation : 0.3% Triton X-100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399