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14-3-3 epsilon antibody

Anti-14-3-3 epsilon antibody used in Western Blot (WB). GTX55485
Anti-14-3-3 epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55485
Anti-14-3-3 epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55485
Anti-14-3-3 epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55485

Cat. No. GTX55485

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human YWHAE (NP_006752.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , 14-3-3E , HEL2 , KCIP-1 , MDCR , MDS

Cellular Localization

Nucleus,Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES

Anti-14-3-3 epsilon antibody used in Western Blot (WB). GTX55485

GTX55485 WB Image

WB analysis of various sample lysates using GTX55485 14-3-3 epsilon antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-14-3-3 epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55485

GTX55485 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55485 14-3-3 epsilon antibody.
Blue : DAPI
Dilution : 1:100

Anti-14-3-3 epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55485

GTX55485 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX55485 14-3-3 epsilon antibody.
Blue : DAPI
Dilution : 1:100

Anti-14-3-3 epsilon antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55485

GTX55485 ICC/IF Image

ICC/IF analysis of L929 cells using GTX55485 14-3-3 epsilon antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399