APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human YWHAE (NP_006752.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon , 14-3-3E , HEL2 , KCIP-1 , MDCR , MDS
Cellular Localization
Nucleus,Cytoplasm
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]
Database
Research Area
DATA IMAGES
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GTX55485 WB Image
WB analysis of various sample lysates using GTX55485 14-3-3 epsilon antibody.
Dilution : 1:3000
Loading : 25μg per lane
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GTX55485 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX55485 14-3-3 epsilon antibody.
Blue : DAPI
Dilution : 1:100
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GTX55485 ICC/IF Image
ICC/IF analysis of H9C2 cells using GTX55485 14-3-3 epsilon antibody.
Blue : DAPI
Dilution : 1:100
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GTX55485 ICC/IF Image
ICC/IF analysis of L929 cells using GTX55485 14-3-3 epsilon antibody.
Blue : DAPI
Dilution : 1:100
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REFERENCE
There are currently no references for 14-3-3 epsilon antibody (GTX55485). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for 14-3-3 epsilon antibody (GTX55485). Be the first to share your experience with this product.
