*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:1000
Not tested in other applications.
This antibody reacts with the human form of intact, non-modified N-terminus 14-3-3 gamma. The naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated is not recognized by this antibody.
Ascites, 0.1% Sodium Azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
N-terminal fragment of human 14-3-3 gamma [UniProt# P61981]
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma , 14-3-3GAMMA , EIEE56 , PPP1R170
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]
GTX48540 WB Image
WB analysis of HeLa (A), A431 (B), NIH-3T3 (C), and PC-12 (D) cell lysate using GTX48540 14-3-3 gamma antibody [HS23].
Dilution : 1:500
GTX48540 WB Image
WB analysis of multiple samples using GTX48540 14-3-3 gamma antibody [HS23].
Lane 1: HeLa cell lysate
Lanes 2 and 3: bengamide treated HeLa cell lysates (8h and 24h, respectively)