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14-3-3 gamma antibody [HS23]

Orthogonal
Anti-14-3-3 gamma antibody [HS23] used in Western Blot (WB). GTX48540
Anti-14-3-3 gamma antibody [HS23] used in Western Blot (WB). GTX48540

Cat. No. GTX48540

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HS23

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Chicken
Package
50 μl ($399)
See all 14-3-3 gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Product Note

This antibody reacts with the human form of intact, non-modified N-terminus 14-3-3 gamma. The naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated is not recognized by this antibody.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

N-terminal fragment of human 14-3-3 gamma [UniProt# P61981]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma , 14-3-3GAMMA , EIEE56 , PPP1R170

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-14-3-3 gamma antibody [HS23] used in Western Blot (WB). GTX48540

GTX48540 WB Image

Orthogonal

WB analysis of multiple samples using GTX48540 14-3-3 gamma antibody [HS23].
Lane 1: HeLa cell lysate
Lanes 2 and 3: bengamide treated HeLa cell lysates (8h and 24h, respectively)

Anti-14-3-3 gamma antibody [HS23] used in Western Blot (WB). GTX48540

GTX48540 WB Image

WB analysis of HeLa (A), A431 (B), NIH-3T3 (C), and PC-12 (D) cell lysate using GTX48540 14-3-3 gamma antibody [HS23].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399