Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using 14-3-3 gamma(GTX110629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma(GTX110629) antibody at 1:200 dilution.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX110629 diluted at 1:10000

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 gamma antibody (GTX110629) diluted at 1:1000 and competitor's antibody (sc-731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX110629 diluted at 1:1000