GeneTex
United States (US)

14-3-3 gamma antibody

Comparison
GTX113298_40128_20161027_WB_competitor_watermark_18121410_338.jpg
GTX113298_40128_IHC_18121410_383.jpg
GTX113298_40128_WB_M_brain_18121410_528.jpg
GTX113298_IFA_18121410_489.jpg
GTX113298_WB_18121410_126.jpg

Cat No. GTX113298

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Gamma,14-3-3Gamma,Eiee56,Ppp1R170,Ywhag

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113298_40128_20161027_WB_competitor_watermark_18121410_338.jpg

GTX113298 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 gamma antibody (GTX113298) diluted at 1:1000 and competitor's antibody (SC-731) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX113298_40128_IHC_18121410_383.jpg

GTX113298 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using 14-3-3 gamma(GTX113298) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113298_40128_WB_M_brain_18121410_528.jpg

GTX113298 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113298 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113298_IFA_18121410_489.jpg

GTX113298 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma(GTX113298) antibody at 1:200 dilution.

GTX113298_WB_18121410_126.jpg

GTX113298 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431 (GTX27909)
12% SDS PAGE
GTX113298 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169