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14-3-3 (phospho Ser58) antibody

Anti-14-3-3 (phospho Ser58) antibody used in Western Blot (WB). GTX82745

Cat. No. GTX82745

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 Human, Rat
Package
100 μl ($499)
See all 14-3-3 products

APPLICATION

Application Note

For WB: use at a dilution of 1:1000.
For IHC: use at a dilution of 1:500.
Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.

Research Area

DATA IMAGES

Anti-14-3-3 (phospho Ser58) antibody used in Western Blot (WB). GTX82745

GTX82745 WB Image

WB analysis of rat brain stem lysates using 14-3-3 (phospho Ser58) antibody in the absence or presence of blocking peptide.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 499