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14-3-3 sigma antibody [1.N.6] (Azide free)

Anti-14-3-3 sigma antibody [1.N.6] (Azide free) used in IHC (Paraffin sections) (IHC-P). GTX14116

Cat. No. GTX14116

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1.N.6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
50 μg ($399)
See all 14-3-3 sigma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1μg/ml for 2 hours at RT
ICC/IF Assay dependent
IHC-P 2-4μg/ml for 30 minutes at RT
IP 2μg antibody/mg protein lysate (Use Protein G)

Note :

IHC-P
Antigen retireval : Requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Product Note

Recognizes human 14-3-3 sigma protein (26-30kD). Does not crossreact with other isoforms of 14-3-3.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human 14-3-3 sigma protein (26-30kD).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SFN , YWHAS , stratifin , 14-3-3 sigma

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-14-3-3 sigma antibody [1.N.6] (Azide free) used in IHC (Paraffin sections) (IHC-P). GTX14116

GTX14116 IHC-P Image

IHC-P analysis of human skin tissue using GTX14116 14-3-3 sigma antibody [1.N.6] (Azide free).

REFERENCE

Clone Reference

Kudo I et al. Oncol Lett 2017; 14 (4):4633-4640 Particular gene upregulation and p53 heterogeneous expression in TP53-mutated maxillary carcinoma.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399