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14-3-3 sigma antibody, Internal

Anti-14-3-3 sigma antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89431
Anti-14-3-3 sigma antibody, Internal used in Western Blot (WB). GTX89431

Cat. No. GTX89431

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($419)
See all 14-3-3 sigma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.03-0.1μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Skin shows strong nuclear staining in selected cells of the basal layer.
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89431-PEP).

Predict Reactivity

Mouse, Rat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stratifin , YWHAS

Cellular Localization

Cytoplasm , Nucleus , Secreted

Background

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-14-3-3 sigma antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89431

GTX89431 IHC-P Image

IHC-P analysis of human skin using GTX89431 14-3-3 sigma antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml

Anti-14-3-3 sigma antibody, Internal used in Western Blot (WB). GTX89431

GTX89431 WB Image

WB analysis of human skin lysate using GTX89431 14-3-3 sigma antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419