GeneTex
United States (US)

14-3-3 sigma antibody

GTX100289_39456_IFA_19011506_665.jpg
GTX100289_39456_IHC_19011506_536.jpg
GTX100289_39456_20160616_WB_19011506_622.jpg
GTX100289_39456_WB_M_19011506_810.jpg
GTX100289_39456_WB_R_19011506_713.jpg
GTX100289_39456_WB_19011506_668.jpg
GTX100289_39456_IHC_M_19011506_299.jpg

Cat No. GTX100289

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , PC-12 , A549

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 14-3-3 sigma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Stratifin,Ywhas,Sfn

Cellular Localization

Cytoplasm , Nucleus , Secreted

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.

Database

Research Area

DATA IMAGES
GTX100289_39456_IFA_19011506_665.jpg

GTX100289 ICC/IF Image

14-3-3 sigma antibody [C1C3] detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold Methanol for 5 min.
Green: 14-3-3 sigma protein stained by 14-3-3 sigma antibody [C1C3] (GTX100289) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX100289_39456_IHC_19011506_536.jpg

GTX100289 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using 14-3-3 sigma(GTX100289) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100289_39456_20160616_WB_19011506_622.jpg

GTX100289 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100289) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100289_39456_WB_M_19011506_810.jpg

GTX100289 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100289) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100289_39456_WB_R_19011506_713.jpg

GTX100289 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100289) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100289_39456_WB_19011506_668.jpg

GTX100289 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100289) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100289_39456_IHC_M_19011506_299.jpg

GTX100289 IHC-P Image

14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
14-3-3 sigma antibody (GTX100289) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169