GeneTex

  • Country / Location Selection

United States (US)

14-3-3 sigma antibody

Orthogonal
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX100289
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289
Anti-14-3-3 sigma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100289
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX100289

Cat. No. GTX100289

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all 14-3-3 sigma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , PC-12 , A549

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 14-3-3 sigma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240391

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stratifin , YWHAS

Cellular Localization

Cytoplasm , Nucleus , Secreted

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.

Database

Research Area

DATA IMAGES

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289

GTX100289 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100289) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289

GTX100289 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100289) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX100289

GTX100289 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using 14-3-3 sigma(GTX100289) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289

GTX100289 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100289) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289

GTX100289 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100289) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100289

GTX100289 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100289) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 sigma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100289

GTX100289 ICC/IF Image

14-3-3 sigma antibody [C1C3] detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold Methanol for 5 min.
Green: 14-3-3 sigma protein stained by 14-3-3 sigma antibody [C1C3] (GTX100289) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX100289

GTX100289 IHC-P Image

14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
14-3-3 sigma antibody (GTX100289) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Wang X et al. Oncol Lett 2021; 22 (1) : 558 Enhanced G1 arrest and apoptosis via MDM4/MDM2 double knockdown and MEK inhibition in wild-type TP53 colon and gastric cancer cells with aberrant KRAS signaling.
Application : WB
Reactivity : Human
Qin X et al. Biochem Biophys Res Commun 2017; (Epub) trim25 is associated with * resistance in non-small-cell lung carcinoma a549-cell line via downregulation of 14-3-3σ.
Application : WB
Reactivity : Human
Chen YJ et al. PLoS One 2011; 6 (3) : e17809 Epstein-Barr virus (EBV) Rta-mediated EBV and Kaposi's sarcoma-associated herpesvirus lytic reactivations in 293 cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for 14-3-3 sigma antibody (GTX100289). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169