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14-3-3 sigma antibody

Orthogonal
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801
Anti-14-3-3 sigma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100801
Anti-14-3-3 sigma antibody used in Immunoprecipitation (IP). GTX100801
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX100801
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX100801
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801
Anti-14-3-3 sigma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100801
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801

Cat. No. GTX100801

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all 14-3-3 sigma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

A549 , 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 sigma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951882

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stratifin , YWHAS

Cellular Localization

Cytoplasm , Nucleus , Secreted

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.

Database

Research Area

DATA IMAGES

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801

GTX100801 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100801) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 sigma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100801

GTX100801 ICC/IF Image

14-3-3 sigma antibody detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 14-3-3 sigma protein stained by 14-3-3 sigma antibody (GTX100801) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-14-3-3 sigma antibody used in Immunoprecipitation (IP). GTX100801

GTX100801 IP Image

14-3-3 sigma antibody immunoprecipitates SFN protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 μg A431 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of SFN protein by 4 μg 14-3-3 sigma antibody (GTX100801)
5 % SDS-PAGE
The immunoprecipitated SFN protein was detected by 14-3-3 sigma antibody (GTX100801) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX100801

GTX100801 IHC-P Image

14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
14-3-3 sigma antibody (GTX100801) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801

GTX100801 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100801) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX100801

GTX100801 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma(GTX100801) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801

GTX100801 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100801) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801

GTX100801 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100801) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 sigma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100801

GTX100801 ICC/IF Image

Orthogonal

The data was published in the journal Oncotarget in 2017. PMID: 29207596

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801

GTX100801 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2017. PMID: 29207596

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801

GTX100801 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2017. PMID: 29207596

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX100801

GTX100801 WB Image

The data was published in the journal Oncotarget in 2017. PMID: 29207596

REFERENCE

Application Reference

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Jingwen Li et al. RSC Adv 2019; 9 (67) : 39082-39089 New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.
Application : WB
Reactivity : Human
Wang C et al. Environ Toxicol Pharmacol 2019; 72 : 103270 Common differentially expressed proteins were found in mouse cleft palate models induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and retinoic acid.
Application : WB
Reactivity : Mouse
Wu JY et al. Anticancer Res 2018; 38 (9) : 5201-5210 Fluoxetine Inhibits DNA Repair and NF-kB-modulated Metastatic Potential in Non-small Cell Lung Cancer.
Application : WB
Reactivity : Human
Kim M et al. Oncotarget 2017; 8 (53) : 90674-90692 UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Jingwen Li et al. RSC Adv 2019; 9 (67):39082-39089 New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.
Application : WB
Reactivity : Human
Wang C et al. Environ Toxicol Pharmacol 2019; 72:103270 Common differentially expressed proteins were found in mouse cleft palate models induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and retinoic acid.
Application : WB
Reactivity : Mouse
Wu JY et al. Anticancer Res 2018; 38 (9):5201-5210 Fluoxetine Inhibits DNA Repair and NF-kB-modulated Metastatic Potential in Non-small Cell Lung Cancer.
Application : WB
Reactivity : Human
Kim M et al. Oncotarget 2017; 8 (53):90674-90692 UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation.
Application : WB
Reactivity : Mouse
Kim M et al. Oncotarget 2017; 8:90674-90692 UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation.
Application : WB
Reactivity : Human
Li Z et al. Stem Cell Res Ther 2015; 6:17 Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium.
Application : ICC/IF
Reactivity : Human
Sinanoglu O et al. Urology 2012; 80 (6):1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.
Application : WB
Reactivity : Mouse
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1):104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Application : WB
Reactivity : Mouse
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169