GeneTex
United States (US)

14-3-3 sigma antibody

GTX100801_39918_IHC_M_19011506_692.jpg
GTX100801_39918_IHC_19011506_923.jpg
GTX100801_39918_WB_M_19011506_437.jpg
GTX100801_40569_IP_19011506_533.jpg
GTX100801_39918_WB_19011506_599.jpg
GTX100801_40569_IFA_19011506_395.jpg
GTX100801_39918_20160728_WB_19011506_790.jpg

Cat No. GTX100801

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

A549 , 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 sigma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

YWHAS antibody, SFN antibody, epithelial cell marker protein 1 antibody, 14-3-3 protein sigma antibody, 14-3-3 sigma antibody, stratifin antibody

Cellular Localization

Cytoplasm , Nucleus , Secreted

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.

Database

Research Area

DATA IMAGES
GTX100801_39918_IHC_M_19011506_692.jpg

GTX100801 IHC-P Image

14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
14-3-3 sigma antibody (GTX100801) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100801_39918_IHC_19011506_923.jpg

GTX100801 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma(GTX100801) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100801_39918_WB_M_19011506_437.jpg

GTX100801 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100801) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100801_40569_IP_19011506_533.jpg

GTX100801 IP Image

14-3-3 sigma antibody immunoprecipitates SFN protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 μg A431 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of SFN protein by 4 μg 14-3-3 sigma antibody (GTX100801)
5 % SDS-PAGE
The immunoprecipitated SFN protein was detected by 14-3-3 sigma antibody (GTX100801) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100801_39918_WB_19011506_599.jpg

GTX100801 WB Image

14-3-3 sigma antibody detects SFN protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
12% SDS-PAGE
14-3-3 sigma antibody (GTX100801) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100801_40569_IFA_19011506_395.jpg

GTX100801 ICC/IF Image

14-3-3 sigma antibody detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 14-3-3 sigma protein stained by 14-3-3 sigma antibody (GTX100801) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX100801_39918_20160728_WB_19011506_790.jpg

GTX100801 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100801) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169