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14-3-3 sigma antibody

Anti-14-3-3 sigma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32418
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX32418
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX32418
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX32418
Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX32418
Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX32418

Cat. No. GTX32418

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all 14-3-3 sigma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SFN (NP_006133.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stratifin , YWHAS

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-14-3-3 sigma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32418

GTX32418 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX32418 14-3-3 sigma antibody.
Blue : DAPI

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX32418

GTX32418 WB Image

WB analysis of various sample lysates using GTX32418 14-3-3 sigma antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX32418

GTX32418 IHC-P Image

IHC-P analysis of rat skin tissue using GTX32418 14-3-3 sigma antibody.
Dilution : 1:100

Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX32418

GTX32418 IHC-P Image

IHC-P analysis of mouse skin tissue using GTX32418 14-3-3 sigma antibody.
Dilution : 1:100

Anti-14-3-3 sigma antibody used in IHC (Paraffin sections) (IHC-P). GTX32418

GTX32418 IHC-P Image

IHC-P analysis of human skin tissue using GTX32418 14-3-3 sigma antibody.
Dilution : 1:100

Anti-14-3-3 sigma antibody used in Western Blot (WB). GTX32418

GTX32418 WB Image

WB analysis of various sample lysates using GTX32418 14-3-3 sigma antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399