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14-3-3 theta antibody [AT1A1]

Anti-14-3-3 theta antibody [AT1A1] used in Western Blot (WB). GTX53680

Cat No. GTX53680

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1A1

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human 14-3-3 theta (1-245aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Theta , 14-3-3 , 1C5 , Hs1 , Ywhaq

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-14-3-3 theta antibody [AT1A1] used in Western Blot (WB). GTX53680

GTX53680 WB Image

WB analysis of Jurkat lysate (20ug) using 14-3-3 tau antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf