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14-3-3 theta antibody

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199
Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199
Anti-14-3-3 theta antibody used in IHC (Paraffin sections) (IHC-P). GTX102199
Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

Cat No. GTX102199

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:30000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , H1299 , HeLaS3 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 theta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta , 14-3-3 , 1C5 , HS1

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq]

Database

DATA IMAGES
Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

GTX102199 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody (GTX102199) diluted at 1:1000 and competitor's antibody (SC-732) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

GTX102199 WB Image

Sample (20 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX102199 diluted at 1:30000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 theta antibody used in IHC (Paraffin sections) (IHC-P). GTX102199

GTX102199 IHC-P Image

14-3-3 theta antibody detects 14-3-3 theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
14-3-3 theta antibody (GTX102199) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

GTX102199 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
12% SDS PAGE
GTX102199 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169