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14-3-3 theta antibody

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX32983
Anti-14-3-3 theta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32983

Cat No. GTX32983

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human YWHAQ (NP_006817.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta , 14-3-3 , 1C5 , HS1

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Anti-14-3-3 theta antibody used in Western Blot (WB). GTX32983

GTX32983 WB Image

WB analysis of various samples using GTX32983 14-3-3 theta antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-14-3-3 theta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32983

GTX32983 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32983 14-3-3 theta antibody.
Blue : DAPI

REFERENCE
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REVIEW
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Package List Price ($)
$ 329