GeneTex
United States (US)

14-3-3 zeta antibody

GTX101075_WB_19011506_640.jpg
GTX101075_WB_M_19011506_150.jpg
GTX101075_IFA_19011506_465.jpg
GTX101075_39834_IHC_M_19011506_463.jpg
GTX101075_39834_IHC_19011506_696.jpg

Cat No. GTX101075

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

14-3-3-zeta antibody, KCIP-1 antibody, MGC111427 antibody, MGC126532 antibody, MGC138156 antibody, YWHAD antibody, YWHAZ antibody, phospholipase A2 antibody, 14-3-3 zeta antibody, protein kinase C inhibitor protein-1 antibody, 14-3-3 delta antibody, 14-3-3 protein zeta/delta antibody, 14-3-3 protein/cytosolic phospholipase A2 antibody, protein kinase C inhibitor protein 1 antibody, "tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide antibody", "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide antibody", "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide antibody"

Cellular Localization

Cytoplasm , Melanosome

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101075_WB_19011506_640.jpg

GTX101075 WB Image

Sample(30 µg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
12% SDS PAGE
GTX101075 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101075_WB_M_19011506_150.jpg

GTX101075 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX101075 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101075_IFA_19011506_465.jpg

GTX101075 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using 14-3-3 zeta (GTX101075) antibody at 1:100 dilution.

GTX101075_39834_IHC_M_19011506_463.jpg

GTX101075 IHC-P Image

14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
14-3-3 zeta antibody (GTX101075) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101075_39834_IHC_19011506_696.jpg

GTX101075 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using 14-3-3 zeta(GTX101075) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169