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14-3-3 zeta antibody

Anti-14-3-3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX101075
Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX101075
Anti-14-3-3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX101075
Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX101075
Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX101075
Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX101075

Cat. No. GTX101075

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all 14-3-3 zeta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

HepG2,Molt-4,NIH-3T3,HeLa,A431,Neuro2A,Rat2

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952637

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta , 14-3-3-zeta , HEL-S-3 , HEL-S-93 , HEL4 , KCIP-1 , YWHAD

Cellular Localization

Cytoplasm , Melanosome

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-14-3-3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX101075

GTX101075 IHC-P Image

14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
14-3-3 zeta antibody (GTX101075) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX101075

GTX101075 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (GTX101075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX101075

GTX101075 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using 14-3-3 zeta(GTX101075) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX101075

GTX101075 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (GTX101075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX101075

GTX101075 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
12% SDS PAGE
GTX101075 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX101075

GTX101075 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX101075 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ferr? A et al. Front Endocrinol (Lausanne) 2023; 14 : 1222724 Neurohypophysial and paracrine vasopressinergic signaling regulates aquaporin trafficking to hydrate marine teleost oocytes.
Liu YJ et al. Neurobiol Dis 2021; 160 : 105531 A system-wide mislocalization of RNA-binding proteins in motor neurons is a new feature of ALS.
Application : WB
Reactivity : Human
Chan TY et al. Nat Commun 2021; 12 (1) : 5337 TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.
Application : WB
Reactivity : Human
Yoon JH et al. Gastric Cancer 2018; (Epub) Gastrokine 1 protein is a potential theragnostic target for gastric cancer.
Application : WB
Reactivity : Human
Brandi J et al. J Proteomics 2016; 136 : 43112 Secretome protein signature of human pancreatic cancer stem-like cells.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169