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14-3-3 zeta antibody

Anti-14-3-3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX50519
Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX50519

Cat No. GTX50519

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.56~60 (R-S-S-W-R) derived from human 14-3-3 zeta.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta , 14-3-3-zeta , HEL-S-3 , HEL-S-93 , HEL4 , KCIP-1 , YWHAD

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-14-3-3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX50519

GTX50519 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50519 14-3-3 zeta antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX50519

GTX50519 WB Image

WB analysis of extracts from HepG2, HeLa, and 293 cells using GTX50519 14-3-3 zeta antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf