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14-3-3 zeta antibody

Orthogonal
Anti-14-3-3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX50519
Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX50519

Cat. No. GTX50519

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all 14-3-3 zeta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.56~60 (R-S-S-W-R) derived from human 14-3-3 zeta.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta , 14-3-3-zeta , HEL-S-3 , HEL-S-93 , HEL4 , KCIP-1 , YWHAD

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-14-3-3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX50519

GTX50519 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50519 14-3-3 zeta antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-14-3-3 zeta antibody used in Western Blot (WB). GTX50519

GTX50519 WB Image

WB analysis of extracts from HepG2, HeLa, and 293 cells using GTX50519 14-3-3 zeta antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399