14-3-3 zeta (phospho Ser58) antibody

Cat. No. GTX50712

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399)

See all 14-3-3 zeta products

Datasheet
Document

View product citations for antibody GTX50712 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:200
IHC-P	1:50-1:100

Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from human 14-3-3 zeta.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta , 14-3-3-zeta , HEL-S-3 , HEL-S-93 , HEL4 , KCIP-1 , YWHAD

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Database

Gene ID: 7534 YWHAZ
UniProt: P63104 YWHAZ

Research Area

DATA IMAGES

GTX50712 WB Image

WB analysis of extracts from HeLa cells untreated or treated with UV using GTX50712 14-3-3 zeta (phospho Ser58) antibody.

GTX50712 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50712 14-3-3 zeta (phospho Ser58) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

GTX50712 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50712 14-3-3 zeta (phospho Ser58) antibody.

REFERENCE

There are currently no references for 14-3-3 zeta (phospho Ser58) antibody (GTX50712). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for 14-3-3 zeta (phospho Ser58) antibody (GTX50712). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty