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15-PGDH antibody [C2C3], C-term

Orthogonal
Anti-15-PGDH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104625
Anti-15-PGDH antibody [C2C3], C-term used in Western Blot (WB). GTX104625
Anti-15-PGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104625
Anti-15-PGDH antibody [C2C3], C-term used in Western Blot (WB). GTX104625

Cat. No. GTX104625

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all 15-PGDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 15-PGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240958

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

15-hydroxyprostaglandin dehydrogenase , 15-PGDH , PGDH , PGDH1 , PHOAR1 , SDR36C1

Background

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-15-PGDH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104625

GTX104625 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 15-PGDH(GTX104625) antibody at 1:200 dilution.

Anti-15-PGDH antibody [C2C3], C-term used in Western Blot (WB). GTX104625

GTX104625 WB Image

Sample (30 μg whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX104625 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-15-PGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104625

GTX104625 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using 15-PGDH(GTX104625) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-15-PGDH antibody [C2C3], C-term used in Western Blot (WB). GTX104625

GTX104625 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 15-PGDH antibody [C2C3], C-term (GTX104625) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169