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15-PGDH antibody

Anti-15-PGDH antibody used in Western Blot (WB). GTX113740
Anti-15-PGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113740
Anti-15-PGDH antibody used in IHC (Paraffin sections) (IHC-P). GTX113740

Cat No. GTX113740

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 15-PGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731270

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

15-hydroxyprostaglandin dehydrogenase , 15-PGDH , PGDH , PGDH1 , PHOAR1 , SDR36C1

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-15-PGDH antibody used in Western Blot (WB). GTX113740

GTX113740 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX113740 diluted at 1:1000

Anti-15-PGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113740

GTX113740 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using 15-PGDH(GTX113740) antibody at 1:500 dilution.

Anti-15-PGDH antibody used in IHC (Paraffin sections) (IHC-P). GTX113740

GTX113740 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using 15-PGDH(GTX113740) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169