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293T whole cell lysate  

293T whole cell lysate  . GTX535673

Cat No. GTX535673

Application

WB

Species

Human
Package
200 μg ($99)
PRODUCT

Summary

Whole-cell lysate of 293T cells prepared in RIPA buffer with no reducing agents and is unboiled.

PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 μg/ml Leupeptin, 2 μg/ml Aprotinin, 1 μg/ml Pepstatin A, 0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

293T whole cell lysate  . GTX535673

GTX535673 WB Image

293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX135012) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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Package List Price ($)
$ 99