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4-1BBL / CD137L antibody [N2C3]

Anti-4-1BBL / CD137L antibody [N2C3] used in Western Blot (WB). GTX117355
Anti-4-1BBL / CD137L antibody [N2C3] used in Immunoprecipitation (IP). GTX117355

Cat. No. GTX117355

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HCT116

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 4-1BBL / CD137L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174321

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF superfamily member 9 , 4-1BB-L , CD137L , TNLG5A

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Database

Research Area

DATA IMAGES

Anti-4-1BBL / CD137L antibody [N2C3] used in Western Blot (WB). GTX117355

GTX117355 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX17355 diluted at 1:1000

Anti-4-1BBL / CD137L antibody [N2C3] used in Immunoprecipitation (IP). GTX117355

GTX117355 IP Image

TNFSF9 antibody [N2C3] immunoprecipitates TNFSF9 protein in IP experiments.
IP samples: A549 membrane extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TNFSF9 protein by 4 μg TNFSF9 antibody [N2C3] (GTX117355)
15 % SDS-PAGE
The immunoprecipitated TNFSF9 protein was detected by TNFSF9 antibody [N2C3] (GTX117355) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

There are currently no references for 4-1BBL / CD137L antibody [N2C3] (GTX117355). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for 4-1BBL / CD137L antibody [N2C3] (GTX117355). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169