4-1BBL / CD137L antibody [N2C3]

Cat. No. GTX117355

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all 4-1BBL / CD137L products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HCT116

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 4-1BBL / CD137L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174321

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF superfamily member 9 , 4-1BB-L , CD137L , TNLG5A

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Database

Gene ID: 8744 TNFSF9
UniProt: P41273 TNFSF9

Research Area

DATA IMAGES

GTX117355 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX17355 diluted at 1:1000

GTX117355 IP Image

TNFSF9 antibody [N2C3] immunoprecipitates TNFSF9 protein in IP experiments.
IP samples: A549 membrane extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TNFSF9 protein by 4 μg TNFSF9 antibody [N2C3] (GTX117355)
15 % SDS-PAGE
The immunoprecipitated TNFSF9 protein was detected by TNFSF9 antibody [N2C3] (GTX117355) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty