GeneTex
United States (US)

4-1BBL / CD137L antibody [N2C3]

GTX117355_40555_WB_18121410_911.jpg
GTX117355_40555_IP_18121410_783.jpg

Cat No. GTX117355

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TNFSF9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Superfamily Member 9,4-1Bb-L,Cd137L,Tnlg5A,Tnfsf9

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Database

Research Area

DATA IMAGES
GTX117355_40555_WB_18121410_911.jpg

GTX117355 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX17355 diluted at 1:1000

GTX117355_40555_IP_18121410_783.jpg

GTX117355 IP Image

TNFSF9 antibody [N2C3] immunoprecipitates TNFSF9 protein in IP experiments.
IP samples: A549 membrane extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TNFSF9 protein by 4 μg TNFSF9 antibody [N2C3] (GTX117355)
15 % SDS-PAGE
The immunoprecipitated TNFSF9 protein was detected by TNFSF9 antibody [N2C3] (GTX117355) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169