GeneTex
United States (US)

4-1BBL / CD137L antibody [N2C3]

GTX117355_40555_WB_18121410_911.jpg
GTX117355_40555_IP_18121410_783.jpg

Cat No. GTX117355

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TNFSF9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

4-1BB-L antibody, CD137L antibody, TNFSF9 antibody, tumor necrosis factor ligand superfamily member 9 antibody, 4-1BBL antibody, receptor 4-1BB ligand antibody, 4-1BB ligand antibody, homolog of mouse 4-1BB-L antibody, "tumor necrosis factor (ligand) superfamily, member 9 antibody"

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Database

Research Area

DATA IMAGES
GTX117355_40555_WB_18121410_911.jpg

GTX117355 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX17355 diluted at 1:1000

GTX117355_40555_IP_18121410_783.jpg

GTX117355 IP Image

TNFSF9 antibody [N2C3] immunoprecipitates TNFSF9 protein in IP experiments.
IP samples: A549 membrane extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TNFSF9 protein by 4 μg TNFSF9 antibody [N2C3] (GTX117355)
15 % SDS-PAGE
The immunoprecipitated TNFSF9 protein was detected by TNFSF9 antibody [N2C3] (GTX117355) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169