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4 Hydroxynonenal antibody [12F7]

Anti-4 Hydroxynonenal antibody [12F7] used in Western Blot (WB). GTX17571
Anti-4 Hydroxynonenal antibody [12F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17571

Cat. No. GTX17571

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 12F7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA

Reactivity

 Species independent

Species

 Species independent
Package
100 μg ($469)
See all 4 Hydroxynonenal products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA 1:1000
Not tested in other applications.

Product Note

Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehy

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic 4-Hydroxynonenal modified Keyhole Limpet Kemocyanin (KLH).

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-4 Hydroxynonenal antibody [12F7] used in Western Blot (WB). GTX17571

GTX17571 WB Image

WB analysis of 4-hydroxy-nonenal-BSA using GTX175714 Hydroxynonenal antibody [12F7].
Lane 1 : Molecular Weight Ladder (MW)
Lane 2 : BSA (0.5 μg)
Lane 3 : 4-hydroxyl nonenal-BSA (0.5 μg)
Lane 4 : 4-hydroxy nonenal-BSA (2.0 μg)
Lane 5 : 4-hydroxy-2-hexenal (0.5 μg)
Lane 6 : 4-hydroxy-2-hexenal (2.0 μg)
Dilution : 1:1000 for 2 hours at RT

Anti-4 Hydroxynonenal antibody [12F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17571

GTX17571 ICC/IF Image

ICC/IF analysis of HEK293 cells treated(B,D,F,H) or untreated(A,C,E,G) with 50 μM H₂O₂ using GTX175714 Hydroxynonenal antibody [12F7].
Green : Primary antibody
Red : F-Actin
Blue : DAPI
Dilution : 1:50 for 30-60 min at RT
Fixation : 5% Formaldehyde for 5 min

REFERENCE

Application Reference

W Zhang et al. J Histotechnol 2023; : 1–15 Study of pathological processes of meibomian gland dysfunction by in vitro culture airlifting conditions
Application : IHC-P
Ma PW et al. Oxid Med Cell Longev 2022; 2022 : 3373828 Treatment with the Ferroptosis Inhibitor Ferrostatin-1 Attenuates Noise-Induced Hearing Loss by Suppressing Ferroptosis and Apoptosis.
Jia-Wei Chen et al. Front Cell Neurosci 2022; 16 : 803718 mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis.
Application : IHC-Fr
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469