Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3-2] (GTX116315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

4E-BP1 antibody [N1C3-2] detects 4E-BP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 4E-BP1 protein stained by 4E-BP1 antibody [N1C3-2] (GTX116315) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody (GTX116315) diluted at a dilution of 1:1000.