GeneTex
United States (US)

4E-BP1 antibody

GTX31714_299_ICC-IF_20180221_18121410_175.jpg
GTX31714_2877_WB_20180221_18121410_268.jpg
GTX31714_298_ICC-IF_20180221_18121410_208.jpg

Cat No. GTX31714

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2.5 - 10 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

3T3 Cell Lysate , 3T3/BALB Cell Slide
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1.The immunogen is located within the last 50 amino acids of 4E-BP1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4E Binding Protein 1,4E-Bp1,4Ebp1,Bp-1,Phas-I,Eif4Ebp1

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31714_299_ICC-IF_20180221_18121410_175.jpg

GTX31714 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31714 4E-BP1 antibody.
Working concentration : 20 μg/ml

GTX31714_2877_WB_20180221_18121410_268.jpg

GTX31714 WB Image

WB analysis of 3T3 cell lysate using GTX31714 4E-BP1 antibody.
Working concentration : (A) 2.5, (B) 5 and (C) 10 μg/ml

GTX31714_298_ICC-IF_20180221_18121410_208.jpg

GTX31714 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31714 4E-BP1 antibody.
Working concentration : 2 μg/ml

Package List Price ($)
$ 329