GeneTex

  • Country / Location Selection

United States (US)

4E-BP1 antibody

Anti-4E-BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31714
Anti-4E-BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31714
Anti-4E-BP1 antibody used in Western Blot (WB). GTX31714

Cat. No. GTX31714

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all 4E-BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2.5 - 10 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

3T3 Cell Lysate , 3T3/BALB Cell Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1.The immunogen is located within the last 50 amino acids of 4E-BP1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E binding protein 1 , 4E-BP1 , 4EBP1 , BP-1 , PHAS-I

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-4E-BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31714

GTX31714 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31714 4E-BP1 antibody.
Working concentration : 20 μg/ml

Anti-4E-BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31714

GTX31714 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31714 4E-BP1 antibody.
Working concentration : 2 μg/ml

Anti-4E-BP1 antibody used in Western Blot (WB). GTX31714

GTX31714 WB Image

WB analysis of 3T3 cell lysate using GTX31714 4E-BP1 antibody.
Working concentration : (A) 2.5, (B) 5 and (C) 10 μg/ml

REFERENCE

There are currently no references for 4E-BP1 antibody (GTX31714). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for 4E-BP1 antibody (GTX31714). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399