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4E-BP1 (phospho Ser65) antibody

Anti-4E-BP1 (phospho Ser65) antibody used in Western Blot (WB). GTX133184
Anti-4E-BP1 (phospho Ser65) antibody used in Western Blot (WB). GTX133184

Cat No. GTX133184

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

HeLa , 293T (starvation for 24 hr) , 293T (starvation for 24 hr and 20%FBS treatment for 0.5 hr)

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human 4E-BP1 (phospho Ser65). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

eukaryotic translation initiation factor 4E binding protein 1,4E-BP1,4EBP1,BP-1,PHAS-I

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-4E-BP1 (phospho Ser65) antibody used in Western Blot (WB). GTX133184

GTX133184 WB Image

Wild-type (WT) and 4E-BP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody (GTX133184) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-4E-BP1 (phospho Ser65) antibody used in Western Blot (WB). GTX133184

GTX133184 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (60 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody (GTX133184) diluted at 1:500.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf